Blood cell analyzer and blood cell analyzing method

• US 9,778,178 B2
• Filed: 03/27/2014
• Issued: 10/03/2017
• Est. Priority Date: 03/29/2013
• Status: Active Grant

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: Yamada, Kazuhiro, Kawashima, Yasuyuki, Tabata, Seiichiro
• Primary Examiner(s): Claytor, Renee
• Assistant Examiner(s): Papciak, Sharon M
• Application Number:
  US14/227,650
• Publication Number:
  US 20140295487A1
• Time in Patent Office: 1,286 Days
• Field of Search: None
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/012 Red blood cells
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/018 Platelets
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1402 Data analysis by thresholding or gating operations performed on the acquired signals or stored data
• CPC Class Codes: G01N 21/49 within a body or fluid

Abstract

A blood cell analyzer comprises a flow cell configured to flow a measurement specimen containing blood cells, a first light source configured to emit light having a first wavelength, a second light source configured to emit light having a second wavelength different from the first wavelength, a first light receiving portion configured to receive first scattered light obtained by irradiating the blood cells passing through the flow cell with light from the first light source, a second light receiving portion configured to receive second scattered light obtained by irradiating the blood cells passing through the flow cell with light from the second light source, and a control section configured to discriminate at least red blood cells from the blood cells contained in the measurement specimen based on detection signals output from the first light receiving portion and the second light receiving portion, respectively.

Claims

1. A blood cell analyzer comprising:

a flow cell configured to flow a measurement specimen containing blood cells therethrough, wherein the flow cell is configured to flow the measurement specimen for a continuous time period that includes without overlap a first period of time and a second period of time;

a first light source configured to irradiate the blood cells flowing through the flow cell with first light having a first wavelength for the continuous time period;

a second light source configured to irradiate the blood cells flowing through the flow cell with second light having a second wavelength longer than the first wavelength for the continuous time period;

a first light receiving portion configured to receive the first light scattered from a respective blood cell and convert the received first light for the continuous time period into a series of first electrical signals carrying first values each representative of an optical property of a respective blood cell exhibited in the first light, wherein the first values comprise a first set of first values obtained for the first period of time and a second set of first values obtained for the second period of time;

a second light receiving portion configured to receive the second light scattered from a respective blood cell and convert the received second light for the continuous time period into a series of second electrical signals carrying second values each representative of an optical property of a respective blood cell exhibited in the second light, wherein the second values comprise a first set of second values obtained for the first period of time and a second set of second values obtained for the second period of time; and

an information processing unit programmed to analyze the first and second values to discriminate red blood cells and white blood cells among the blood cells contained in the measurement specimen, the information processing unit being programmed to;

apply a first threshold to the first pair of correlated first and second values of the blood cells to exclude the first and second values representative of the signal noise from the first pair of correlated first and second values of the blood cells;

correlate the first sets of first and second values to each other to characterize a respective blood cell with a first pair of correlated first and second values, wherein the first and second wavelengths are selected such that if plotted in a two-dimensional plane defined by first and second orthogonal coordinates representative, respectively, of the first pair of correlated first and second values, a cluster of red blood cells and a cluster of white blood cells are separately distributed in the two-dimensional plane with a distance;

store the first pair of correlated first and second values in a memory for a respective blood cell;

analyze the first pair of correlated first and second values of the blood cells stored in the memory to identify a group of blood cells in the measurement specimen having first and second values that fall within a first region of the two-dimensional plane representative of the cluster of red blood cells plotted in the two-dimensional plane;

apply a second threshold, different from the first threshold, to the second pair of correlated first and second values of the blood cells to exclude the first and second values representative of the red blood cells from the second pair of correlated first and second values of the blood cells;

correlate the second sets of first and second values to each other to characterize a respective blood cell with a second pair of correlated first and second values;

store in the memory the second pair of correlated first and second values of the blood cells, wherein the second pair of correlated first and second values representative of red blood cells are excluded; and

analyze the second sets of correlated first and second values of the blood cells stored in the memory to identify a group of blood cells in the measurement specimen having first and second values that fall within a second region in the two-dimensional plane representative of the cluster of white blood cells plotted in the two-dimensional plane.

2. The blood cell analyzer according to claim 1, wherein the first and second wavelengths are selected such that the cluster of red blood cells and a cluster of blood platelets are separately distributed in the two-dimensional plane.

3. The blood cell analyzer according to claim 1, wherein an absorption coefficient of hemoglobin of the first wavelength is different from an absorption coefficient of hemoglobin of the second wavelength.

4. The blood cell analyzer according to claim 1, wherein the first light source is a semiconductor laser light source, and the first wavelength is selected from a range between 400 nm and 435 nm.

5. The blood cell analyzer according to claim 1, wherein the second light source is a semiconductor laser light source, and the second wavelength is selected from a range between 610 nm and 750 nm.

6. The blood cell analyzer according to claim 1, wherein the first light scattered from a respective blood cell is a forward scattered light, and the second light scattered from a respective blood cell is a forward scattered light.

7. The blood cell analyzer according to claim 1, wherein the first light receiving portion is configured to convert intensities of the received first light into the series of first electrical signals, and the second light receiving portion is configured to convert intensities of the received second light into the series of second electrical signals.

8. The blood cell analyzer according to claim 7, further comprising a display section configured to display an image, wherein the information processing unit is programmed to project the two-dimensional plane on the display that shows the cluster of red blood cells and the cluster of white blood cells.

9. The blood cell analyzer according to claim 1, wherein the information processing unit is further programmed to analyze the first and second values of the group of blood cells identified as the cluster of white blood cells and identify subgroups of the first and second values representative, respectively, of subcategories of white blood cells.

10. A blood cell analyzing method comprising:

flowing a measurement specimen containing blood cells through a flow cell for a continuous time period that includes without overlap a first period of time and a second period of time;

irradiating the blood cells flowing through the flow cell with first light having a first wavelength for the continuous time period;

receiving the first light scattered from a respective blood cell and converting the received first light for the continuous time period into a series of first electrical signals carrying first values each representative of an optical property of a respective blood cell exhibited in the first light, wherein the first values comprise a first set of first values obtained for the first period of time and a second set of first values obtained for the second period of time;

irradiating the blood cells flowing through the flow cell with second light having a second wavelength longer than the first wavelength for the continuous time period;

receiving the second light scattered from a respective blood cell and converting the received second light for the continuous time period into a series of second electrical signals carrying second values each representative of an optical property of a respective blood cell exhibited in the second light, wherein the second values comprise a first set of second values obtained for the first period of time and a second set of second values obtained for the second period of time; and

analyzing the first and second values to discriminate red blood cells and white blood cells among the blood cells contained in the measurement specimen, wherein the analyzing the first and second values to discriminate red blood cells and white blood cells among the blood cells comprises;

applying a first threshold to the first pair of correlated first and second values of the blood cells to exclude the first and second values representative of the signal noise from the first pair of correlated first and second values of the blood cells;

correlating the first sets of first and second values to each other to characterize a respective blood cell with a first pair of correlated first and second values, wherein the first and second wavelengths are selected such that if plotted in a two-dimensional plane defined by first and second orthogonal coordinates representative, respectively, of the first pair of correlated first and second values, a cluster of red blood cells and a cluster of white blood cells are separated distributed in the two-dimensional plane with a distance;

storing the first pair of correlated first and second values in a memory for a respective blood cell;

analyzing the first pair of correlated first and second values of the blood cells stored in the memory to identify a group of blood cells in the measurement specimen having first and second values that fall within a first region of the two-dimensional plane representative of the cluster of red blood cells plotted in the two-dimensional plane;

applying a second threshold, different from the first threshold, to the second pair of correlated first and second values of the blood cells to exclude the first and second values representative of the red blood cells from the second pair of correlated first and second values of the blood cells;

correlating the second sets of first and second values to each other to characterize a respective blood cell with a second pair of correlated first and second values,

storing in the memory the second pair of correlated first and second values of the blood cells, wherein the second pair of correlated first and second values representative of red blood cells are excluded; and

analyzing the second sets of correlated first and second values of the blood cells stored in the memory to identify a group of blood cells in the measurement specimen having first and second values that fall within a second region in the two-dimensional plane representative of the cluster of white cells plotted in the two-dimensional plane.

11. The blood cell analyzing method according to claim 10, wherein the first and second wavelengths are selected such that the cluster of red blood cells and a cluster of blood platelets are separately distributed in the two-dimensional plane.

12. The blood cell analyzing method according to claim 10, wherein an absorption coefficient of hemoglobin of the first wavelength is different from an absorption coefficient of hemoglobin of the second wavelength.

13. The blood cell analyzing method according to claim 10, wherein the first wavelength is selected from a range between 400 nm and 435 nm.

14. The blood cell analyzing method according to claim 10, wherein the second wavelength is selected from a range between 610 nm and 750 nm.

15. The blood cell analyzing method according to claim 10, wherein the first light scattered from a respective blood cell is a forward scattered light, and the second light scattered from a respective blood cell is a forward scattered light.

16. The blood cell analyzing method according to claim 10, wherein

converting the received first light into a series of first electrical signals comprises converting intensities of the received first light into a series of first electrical signals, and

converting the received second light into a series of second electrical signals comprises converting intensities of the received second light into a series of second electrical signals.

17. The blood cell analyzing method according to claim 10, further comprising analyzing the first and second values of the group of blood cells identified as the cluster of white blood cells and identifying subgroups of the first and second values representative, respectively, of subcategories of white blood cells.