Compact sensor for measuring turbidity or fluorescence in a fluid sample

• US 9,778,180 B2
• Filed: 11/10/2015
• Issued: 10/03/2017
• Est. Priority Date: 11/10/2014
• Status: Active Grant

• Current Assignee: In-Situ Incorporated
• Original Assignee: In-Situ Incorporated
• Inventors: Baltz, Nathan T., Sewell, Steven Collin
• Primary Examiner(s): Geisel, Kara E
• Assistant Examiner(s): BOLOGNA, DOMINIC JOSEPH
• Application Number:
  US14/937,240
• Publication Number:
  US 20160139046A1
• Time in Patent Office: 693 Days
• CPC Class Codes: G01N 21/532 with measurement of scattering and transmission
• CPC Class Codes: G01N 2201/068 Optics, miscellaneous
• CPC Class Codes: G01N 27/07 Construction of measuring vessels; Electrodes therefor
• CPC Class Codes: G01N 33/18 Water

Abstract

Provided are turbidometers and fluorometers having a unique form-factor to accommodate a number of optical components in a confined geometry. This provides the ability to compensate for change in light intensity from an optical source even in a closed-loop manner. The ability to package reference and signal detectors, along with a relatively large diameter LED light source in a confined geometry is particularly suited for applications requiring small-diameter sensors, such as multi-parameter sonde devices having a total diameter that is in the sub-two inch range.

Claims

1. A sensor for measuring turbidity or fluorescence comprising:

a distal sensing end having;

a vertex region;

a first surface extending from said vertex region and ending at a first surface end point;

a second surface extending from said vertex region and ending at a second surface end point, wherein said first surface and said second surface extend in different directions to form a vertex angle at said vertex region;

a convex-curved outer surface that extends between said first surface end point and said second surface end point;

a distal sensing surface defined by edges of said first surface, said second surface, said vertex region, and said curved outer surface;

wherein a separation distance between said first surface end point and said second surface end point defines a maximum straight line distance on said distal sensing surface;

a distal sensing end volume having a cross-section formed by said vertex region, said first surface, said second surface and said curved outer surface;

an optical system positioned in said distal sensing end volume comprising;

an optical source to generate a beam of electromagnetic radiation;

an emission window through said distal sensing surface in optical communication with said beam of electromagnetic radiation and configured to pass at least a portion of said beam of electromagnetic radiation from said distal sensing end volume through said distal sensing surface to a sample volume adjacent to said distal sensing surface in an excitation direction;

a collection window through said distal sensing surface configured to pass at least a portion of an incoming beam of electromagnetic radiation in a collection direction to said distal sensing end volume, wherein said excitation direction and said collection direction define a measurement angle that is within 5°

of a right angle; and

a signal photodetector configured to detect said beam of incoming electromagnetic radiation in said collection direction;

wherein said optical system is positioned in an optical plane that extends substantially perpendicular to said distal sensing surface and that is substantially aligned with a notional line corresponding to said maximum straight line distance.

2. The sensor of claim 1, wherein said optical system further comprises:

a beam splitter positioned in optical communication with said light source; and

a reference photodetector to detect electromagnetic radiation reflected by said beam splitter.

3. The sensor of claim 1, wherein said distal sensing surface has a surface area that is less than or equal to 4 cm² and said maximum straight line distance is greater than or equal to 2.3 cm.

4. The sensor of claim 1, wherein said vertex angle is greater than or equal to 30°

and less than 180°

.

5. The sensor of claim 4, wherein said outer surface has a radius of curvature that is less than or equal to 3 cm and said optical system is positioned within 5 cm from said distal sensing surface.

6. The sensor of claim 1, wherein said optical source and said photodetector are configured to provide a sensing height from said distal sensing surface of between 1 mm and 3.6 mm and a sensing volume of between 10 mm³ and 30 mm³.

7. The sensor of claim 6, wherein said optical source and said photodetector are configured to provide a nominal optical path length between said optical source and said photodetector in water that is between 4 mm and 10 mm.

8. The sensor of claim 6, having a dynamic range that spans up to 10,000 NTU.

9. The sensor of claim 1, wherein said first surface and said second surface are flat-faced and said sensor is configured for insertion into a multiparameter sonde comprising a plurality of independent sensors, wherein the sensors in combination provide a substantially circular cross-sectional footprint, and said vertex region comprises a notch for accommodating at least a portion of a drive shaft.

10. The sensor of claim 1, wherein said distal sensing end volume cross-section has a cross-sectional shape formed by:

said first inner surface and said second inner side that are straight-line linear having a length that is greater than or equal to 1.5 cm and less than or equal to 3 cm, and form a vertex angle with respect to each other that is greater than or equal to 30° and

less than or equal to 60°

;

said outer surface having a shape that is curved; and

an interior-facing vertex side that is curved for accommodating a portion of a rotatable drive shaft.

11. The sensor of claim 1, wherein said optical source is a LED having a diameter that is greater than or equal to 5 mm.

12. The sensor of claim 2, wherein said beam splitter is comprised of a material selected from the group consisting of:

sapphire, LASF9 glass, quartz, BK7, clear plastic, polycarbonate, cyclic olefin copolymer and acrylic.

13. The sensor of claim 1, wherein said beam splitter comprises a top surface facing said emission window and a bottom surface facing said optical surface, wherein one or both of said top and bottom surfaces are coated with an optical coating layer.

14. The sensor of claim 2, further comprising an adjustable aperture optically connected to said reference photodetector to control light intensity to said reference photodetector.

15. The sensor of claim 1, further comprising one or more optical filters for controlling wavelength of transmitted electromagnetic radiation.

16. The sensor of claim 1, comprising optical sources and detectors having integrated lenses, filters, or lenses and filters.

17. The sensor of claim 1, wherein said windows comprise a window material selected from the group consisting of a refraction material having an index of refraction that is greater than or equal to 1.7 over a wavelength range between 820 nm and 900 nm, wherein said window material is selected from the group consisting of:

sapphire;

LASF9 glass, clear plastic, polycarbonate, cyclic olefin copolymer, and acrylic.

18. The sensor of claim 17, further comprising an emission optical axis corresponding to an alignment direction of said optical source and a detection optical axis corresponding to an alignment direction of said signal photodetector, wherein an optical angle formed by said emission optical axis and said detection optical axis is less than 70°

.

19. The sensor of claim 17, further comprising a wedge window formed of a material having an index of refraction that is greater than or equal to 1.7 for a wavelength range that is between 820 nm and 900 nm, wherein said wedge window material is adhered to said emission window material or said collection window material.

20. The sensor of claim 17, further comprising an end cap through which said emission and collection windows traverse, wherein said window material forms a crevice-free connection with said distal sensing end to facilitate surface cleaning and minimize unwanted biological growth during use in a liquid environment, wherein said window material covers both said emission window and said collection window.

21. The sensor of claim 20, further comprising a light trap connected to said end cap to attenuate unwanted internally reflected electromagnetic radiation.

22. The sensor of claim 1, further comprising:

a sensor guard operably connected to said distal sensing end, wherein said sensor guard comprises an inner surface having a black coating that defines said sample chamber;

a plurality of passages through said sensor guard for introducing a liquid sample to said distal sensing end;

wherein said guard inner surface and plurality of passages are configured to provide an optically uniform surface to minimize effect of guard orientation on an intensity of said beam of incoming electromagnetic radiation.

23. The sensor of claim 2, wherein a ratio of light intensity detected by said reference detector and said signal detector compensates for a temperature-induced variation in optical output from said light source, thereby providing temperature compensation without a temperature measurement.

24. The sensor of claim 2, wherein a ratio of light intensity detected by said reference detector to said light intensity detected by said signal detector is independent of light intensity generated by said light source.

25. The sensor of claim 1, with a long-term stability characterized by a sensor output drift that is less than 0.5% per year.

26. The sensor of claim 1 that is a turbidity sensor having a dynamic range of at least 4000 NTU.

27. The sensor of claim 1 that is a fluorescent sensor.

28. The sensor of claim 1, further comprising a block that is optically opaque to visible light in which said signal and reference photodetectors are embedded, further comprising a light path through said block to provide optical communication between said light source and said signal and reference photodetectors.

29. A method of measuring turbidity or fluorescence in a fluid sample by:

providing a sensor of claim 1;

introducing a fluid sample to said distal sensing end;

introducing electromagnetic radiation to said fluid sample from said optical source;

detecting a reference light intensity with said reference photodetector;

detecting a signal light intensity with said signal photodetector; and

calculating a ratio of said reference and signal light intensity;

thereby measuring turbidity or fluorescence in said fluid sample.