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Ratiometric immunoassay method and blood testing device

  • US 9,778,251 B2
  • Filed: 05/01/2015
  • Issued: 10/03/2017
  • Est. Priority Date: 12/03/2010
  • Status: Active Grant
First Claim
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1. A method of performing an immunoassay in a blood sample, comprising:

  • introducing a blood sample into a sample entry port of a cartridge and into a sample holding chamber oriented between said sample entry port and a sample extraction unit, wherein a distal portion of said extraction unit defines a metered volume of sample for dilution;

    loading said extraction unit with said sample;

    expelling a diluent into a diluent conduit oriented between a diluent entry port and a diluent introduction port and having an air introduction port disposed therebetween for introduction of air into the diluent conduit, wherein a volume within the diluent conduit between the air introduction port and the diluent introduction port defines the volume of the diluent;

    introducing air into the diluent conduit via the air introduction port to pump the volume of the diluent through the diluent introduction port and over and/or through the sample extraction unit and to wash a portion of said sample from the sample extraction unit to form a diluted sample;

    transferring said diluted sample to a sensing region comprising a first sensor and a second sensor, said first sensor comprising an immunosensor for a first analyte and said second sensor comprising an immunosensor for a second analyte;

    forming a first sandwich on said first sensor comprising an immobilized first antibody, the first analyte and a first labeled antibody;

    forming a second sandwich on said second sensor comprising an immobilized second antibody, the second analyte and a second labeled antibody;

    washing said diluted sample from the sensing region;

    generating a first signal from the first labeled antibody;

    generating a second signal from the second labeled antibody;

    detecting the first signal; and

    ,detecting the second signal.

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