Tube for measuring bio-related substance and quantifying system

• US 9,778,253 B2
• Filed: 04/20/2010
• Issued: 10/03/2017
• Est. Priority Date: 04/20/2009
• Status: Active Grant

• Current Assignee: Universal Bio Research Co., Ltd (Precision System Science Co., Ltd)
• Original Assignee: Universal Bio Research Co., Ltd (Precision System Science Co., Ltd)
• Inventors: Tajima, Hideji
• Primary Examiner(s): Chin, Chris L
• Application Number:
  US13/265,078
• Publication Number:
  US 20120094307A1
• Time in Patent Office: 2,723 Days
• Field of Search: 436501, 422549, 422 69
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form

Abstract

A bio-related substance assay tube 2 comprises a target substance capture bead 3 serving as a first microparticle, compensation bead 4 serving as a second microparticle on which a given amount of a bio-related substance has been immobilized, and a negative control bead 5 serving as a third microparticle for use as a negative control. A mount unit 12 comprises a nozzle communicating with a pump, and the bio-related substance assay tube 2 is mounted in the mount unit 12 to ensure communication with this nozzle. An analyte is introduced into the bio-related substance assay tube 2, followed by labeling the bio-related substance bound to each microparticle to cause light emission. Based on light emission from each microparticle, a calibration curve is prepared or the emission intensity of the first microparticle is compensated to quantify the bio-related substance.

Claims

1. A quantification system for a bio-related substance, which comprises:

a bio-related substance assay tube;

a detection unit for detecting signals emitted from microparticles in the tube;

wherein the microparticles comprises;

a first microparticle on which a substance capable of binding to a target bio-related substance to be measured is immobilized, the first microparticle fixed in a first known position in the tube;

a second microparticle on which the bio-related substance is immobilized in a given amount, the second microparticle fixed in a second known position in the tube; and

a third microparticle for use as a negative control, the third microparticle fixed in a third known position in the tube,

wherein these microparticles are aligned along the longitudinal direction of the tube, and

wherein the detection unit moves relative to the tube along the longitudinal direction of the tube.

2. The quantification system according to claim 1, wherein the second microparticle is a microparticle used for compensation of data measured for the bio-related substance.

3. The quantification system according to claim 1, wherein the second microparticle comprises a plurality of microparticles on which different amounts of the bio-related substance are immobilized.

4. The quantification system according to claim 3, wherein the plurality of microparticles are microparticles used for preparation of a calibration curve.

5. The quantification system according to any one of claims 1 to 3, which further comprises light-shielding members interposed to separate the first to third microparticles.

6. The quantification system according to claim 1, which further comprises:

a compensation unit for compensating data of the detected signals by referring to a calibration curve which has been prepared; and

an arithmetic unit for quantifying the bio-related substance based on the compensated data.

7. The quantification system according to claim 1, which further comprises:

a calibration curve preparation unit for preparing a calibration curve based on the detected signals; and

an arithmetic unit for quantifying the bio-related substance by referring to the prepared calibration curve.

8. A method for measuring a bio-related substance by using the quantification system according to claim 1, which comprises contacting an analyte with the tube, and measuring the target bio-related substance in the analyte.

9. The quantification system according to claim 1, wherein the first, second and third microparticles are aligned one by one in line.

10. The quantification system according to claim 1, further comprising a light irradiation unit, the light irradiation unit and a light receiving unit are controlled for their movement.