Methods for identifying modulators of ion channels
First Claim
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1. A method of identifying a compound that is a modulator of an ion channel comprising:
- (a) applying cells to a first location and a second location on a surface of colorimetric resonant reflectance optical biosensor;
(b) applying a test reagent compound to the first location;
(c) applying a compound that is a known ion channel modulator of the cells to the second location;
(d) detecting a colorimetric resonant reflectance optical first peak wavelength value (PWV) for the first location and detecting a second PWV for the second location;
wherein if the first and second PWVs are the same or similar, then the test reagent compound is a modulator of an ion channel; and
wherein if the first and second PWVs are substantially different, then the test reagent is not a compound that is a modulator of an ion channel.
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Abstract
The invention provides methods for identifying modulators of ion channels without the use of recombinant cell lines over-expressing the ion channel proteins or the use of detection labels.
251 Citations
20 Claims
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1. A method of identifying a compound that is a modulator of an ion channel comprising:
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(a) applying cells to a first location and a second location on a surface of colorimetric resonant reflectance optical biosensor; (b) applying a test reagent compound to the first location; (c) applying a compound that is a known ion channel modulator of the cells to the second location; (d) detecting a colorimetric resonant reflectance optical first peak wavelength value (PWV) for the first location and detecting a second PWV for the second location;
wherein if the first and second PWVs are the same or similar, then the test reagent compound is a modulator of an ion channel; and
wherein if the first and second PWVs are substantially different, then the test reagent is not a compound that is a modulator of an ion channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of identifying a compound that is a modulator of an ion channel comprising:
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(a) applying cells to a surface of colorimetric resonant reflectance optical biosensor; (b) applying a test reagent compound to the cells; (c) detecting a first colorimetric resonant reflectance optical peak wavelength value (PWV) pattern for the cells over time and comparing the first PWV pattern for the cells over time to a second PWV pattern for cells treated with a known ion channel modulator over time, wherein if the first and second PWV patterns are the same or similar, then the test reagent compound is a modulator of an ion channel.
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Specification