Isolated communication sample processing system and methods of biological slide processing

• US 9,778,273 B2
• Filed: 12/22/2003
• Issued: 10/03/2017
• Est. Priority Date: 12/20/2002
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Dako Denmark A/S (Agilent Technologies, Inc.)
• Inventors: Feingold, Gordon, Key, Marc
• Primary Examiner(s): Gordon, Brian R
• Application Number:
  US10/539,192
• Publication Number:
  US 20060088940A1
• Time in Patent Office: 5,034 Days
• Field of Search: 422 99-100, 422 63- 681, 422501, 422519, 700266, 436180
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00039 Transport arrangements specific to flat sample substrates, e.g. pusher blade
• CPC Class Codes: G01N 2035/00089 Magazines
• CPC Class Codes: G01N 2035/00306 Housings, cabinets, control panels (details)
• CPC Class Codes: G01N 2035/00326 Analysers with modular structure
• CPC Class Codes: G01N 2035/00366 Several different temperatures used
• CPC Class Codes: G01N 2035/00376 Conductive heating, e.g. heated plates
• CPC Class Codes: G01N 2035/00435 Refrigerated reagent storage
• CPC Class Codes: G01N 2035/00881 network configurations
• CPC Class Codes: G01N 2035/00891 Displaying information to the operator
• CPC Class Codes: G01N 2035/009 alarms, e.g. audible
• CPC Class Codes: G01N 2035/0091 GUI [graphical user interfaces]
• CPC Class Codes: G01N 35/00712 Automatic status testing, e.g. at start-up or periodic
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: G01N 35/00871 Communications between instruments or with remote terminals
• CPC Class Codes: G01N 35/0092 Scheduling
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G16H 50/20 for computer-aided diagnosis, e.g. based on medical expert systems
• CPC Class Codes: Y02A 90/10 : Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
• CPC Class Codes: Y10S 901/02 : Arm motion controller
• CPC Class Codes: Y10S 901/41 : Tool
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis
• CPC Class Codes: Y10T 436/112499 : with sample on test slide
• CPC Class Codes: Y10T 436/113332 : with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114165 : with step of insertion or removal from test line
• CPC Class Codes: Y10T 436/114998 : with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/25 : including sample preparation
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A sample processing system 101 that may be automated and methods are disclosed where a number of sample processing systems 101, such as stainer, may be connected to a number of separate full function computers 181 through a stainer network 183 that may be isolated from other communication traffic. A network configuration may permit scalability and addressability so that additional sample processing systems 101, additional separate full function computers 181, and additional other devices such as label printers 200 may be easily added to the system. One or more remote information links 171 may be provided so that information transfer on a continuous or perhaps constant basis can be accommodated.

Claims

1. A method for performing operations on a stainer in a stainer network comprising:

providing a robotic arm coupled to the stainer, the robotic arm having a reagent dispenser;

establishing a network connection between a computer and a stainer in the stainer network;

sending requests from the computer to the stainer over the network connection;

inserting a first sample into the stainer;

processing a first sample with the stainer using the requests received from the computer, wherein said processing comprises dispensing reagents to the sample with the reagent dispenser of the robotic arm; and

inserting a second sample into the stainer and at least one reagent into the stainer during the processing of the first sample without interrupting the dispensing of reagents onto the first sample with the reagent dispenser of the robotic arm.

2. The method of claim 1, wherein the computer is a server and wherein the stainer is a client of the server.

3. The method of claim 2, wherein the server includes a centralized database including configuration information for the stainer.

4. The method of claim 1, wherein the stainer network is connected to a laboratory information system.

5. The method of claim 4, further including sending requests from the laboratory information system to a server including a centralized database comprising information about the stainer, wherein the information includes status information on stainers, slides, consumables, and treatment protocols of the stainer.

6. The method of claim 5, wherein the requests from the laboratory information system to the server including the centralized database further comprise database maintenance operations including information purging, information compaction, and database information back-up operations.

7. The method of claim 3, further comprising:

sending a request from the computer to run diagnostic tests on the stainer,

storing diagnostic information in the centralized database,

retrieving the diagnostic information about the stainer from the centralized database by the computer; and

providing the diagnostic information to a user.

8. The method of claim 7, further comprising exercising active components of the stainer as a diagnostic test, the active components including at least one of the robotic arm, the reagent dispenser, an optical sensor, an actuator probe, and a non-discrete applicator.

9. The method of claim 8, further comprising electronically notifying an operator about the results of the diagnostic tests.

10. The method of claim 1, further comprising performing automatic diagnostic operations on the stainer while operating other stainers in the stainer network, wherein the operations of the other stainers are not materially affected by the operations of the stainer.

11. The method of claim 1, further comprising monitoring by the computer a status of at least one of the first sample or the second sample in the stainer.

12. The method of claim 1, further comprising providing by the computer a real-time estimate of a completion time of the first sample being processed by the stainer.

13. A method for performing operations on a stainer in a stainer network comprising:

providing a robotic arm coupled to the stainer, the robotic arm having a reagent dispenser;

establishing a network connection between a computer and a stainer in the stainer network;

sending requests from the computer to the stainer over the network connection;

sending requests from the laboratory information system to a server including a centralized database, wherein the centralized database contains status information chosen from slides on the stainer, amounts of consumables on the stainer or treatment protocols performed on the stainer;

inserting a first sample into the stainer;

processing a first sample with the stainer using the requests received from the computer, wherein said processing comprises dispensing reagents to the sample with the reagent dispenser of the robotic arm; and

inserting a second sample into the stainer and at least one reagent into the stainer during the processing of the first sample without interrupting the dispensing of reagents onto the first sample with the reagent dispenser of the robotic arm.

14. The method of claim 13, wherein the stainer network further includes a server and a plurality of stainers including the stainer, and wherein the stainer is a client of the server.

15. The method of claim 14, wherein the server includes a centralized database comprising configuration information for the stainer.

16. The method of claim 13, wherein the requests further comprise database maintenance operations including information purging, information compaction, and database information.

17. The method of claim 13, wherein the stainer is a first stainer,

the method further comprising:

sending requests from the computer to the stainer over the network connection according to an event topology;

providing a second stainer in the stainer network;

checking by the computer for operational readiness in the first stainer; and

altering the event topology according to the operational readiness.

18. The method of claim 17, further comprising:

sending requests from the computer to run diagnostic tests on the first or the second stainer,

storing diagnostic information in the centralized database,

retrieving diagnostic information about the first or the second stainer from the centralized database by the computer; and

providing the diagnostic information to a user.

19. The method of claim 18, further comprising exercising active components of the first or the second stainer as a diagnostic test, the active components including at least one of a robotic arm of the first or the second stainer, a reagent dispenser of the first or the second stainer, an optical sensor of the first or the second stainer, an actuator probe of the first or the second stainer, and a non-discrete applicator of the first or the second stainer.

20. The method of claim 19, further comprising electronically notifying an operator about the results of the diagnostic tests.

21. The method of claim 13, wherein the stainer is configured to be controlled from a remote location.

22. The method of claim 13, further comprising performing automatic diagnostic operations on the stainer while operating other stainers in the stainer network, wherein the operations of the other stainers are not materially affected by the operations of the stainer.

23. The method of claim 13, further comprising monitoring by the computer the status of at least one of the first sample or the second sample in the stainer.

24. The method of claim 13, further comprising providing by the computer a real-time estimate of the completion time of the first sample being processed by the stainer.