Automated prostate tissue referencing for cancer detection and diagnosis
First Claim
Patent Images
1. A method of identifying prostate tissue samples, comprising:
- obtaining, by a processor, a Hematoxylin and Eosin (HE) stained image of a test prostate tissue sample;
obtaining, by the processor, an Infrared (IR) image of the test prostate tissue sample;
performing segmentation, by the processor, of lumens and nuclei in the HE stained image and the IR image of the test prostate tissue sample;
identifying, by the processor, cells, cell nuclei and lumens using the HE stained image and the IR image according to the segmentation;
selecting, by the processor, a group of morphological features from a plurality of morphological features extracted from the HE stained image and the IR image of the test prostate tissue sample identified according to the segmentation, by utilizing a minimum-redundancy-maximal-relevance (mRMR) criterion applied by the processor;
determining, by the processor, similarities between the group of morphological features extracted from the test prostate tissue sample and corresponding morphological features extracted from a plurality of prostate tissue samples, wherein HE images and IR images for the plurality of prostate tissue samples and plural morphological features extracted from the plurality of prostate tissue samples are stored in a database;
retrieving, by the processor from the database, HE images and IR images of one or more prostate tissue samples in the plurality of prostate tissue samples that are most similar to the test prostate tissue sample based on the group of morphological features selected; and
displaying the HE images and IR images of the one or more prostate tissue samples retrieved.
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Abstract
This application provides to a method for identifying one or more prostate tissue samples in a database that are closest to a test prostate sample, which can be used to aid pathologists when examining prostate tissues to attain reliable and consistent diagnoses of prostate cancer. Also provided are databases and computer algorithms that can be used with such methods.
19 Citations
19 Claims
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1. A method of identifying prostate tissue samples, comprising:
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obtaining, by a processor, a Hematoxylin and Eosin (HE) stained image of a test prostate tissue sample; obtaining, by the processor, an Infrared (IR) image of the test prostate tissue sample; performing segmentation, by the processor, of lumens and nuclei in the HE stained image and the IR image of the test prostate tissue sample; identifying, by the processor, cells, cell nuclei and lumens using the HE stained image and the IR image according to the segmentation; selecting, by the processor, a group of morphological features from a plurality of morphological features extracted from the HE stained image and the IR image of the test prostate tissue sample identified according to the segmentation, by utilizing a minimum-redundancy-maximal-relevance (mRMR) criterion applied by the processor; determining, by the processor, similarities between the group of morphological features extracted from the test prostate tissue sample and corresponding morphological features extracted from a plurality of prostate tissue samples, wherein HE images and IR images for the plurality of prostate tissue samples and plural morphological features extracted from the plurality of prostate tissue samples are stored in a database; retrieving, by the processor from the database, HE images and IR images of one or more prostate tissue samples in the plurality of prostate tissue samples that are most similar to the test prostate tissue sample based on the group of morphological features selected; and displaying the HE images and IR images of the one or more prostate tissue samples retrieved. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A system, comprising:
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a microscopic imaging device; an infrared (IR) imaging device; a memory that stores executable instructions; and a processor coupled with the memory, wherein the processor, responsive to executing the instructions, performs operations comprising; capturing a digitized optical image of a Hematoxylin and Eosin (HE) stained reference sample of prostate tissue with the microscopic imaging device; obtaining a digitized IR image of the reference sample of prostate tissue with the IR imaging device; registering the digitized optical image and the digitized IR image, thereby forming a composite image; segmenting the composite image into epithelium nuclei and lumen; extracting a plurality of morphologic feature information from the segmented composite image; storing the extracted plurality of morphologic feature information in a database; selecting a proper subset of morphologic features from the plurality of morphologic feature information; and determining a similarity by comparing the stored extracted plurality of morphologic feature information of the proper subset of morphologic features with corresponding morphologic feature information from a test sample of prostate tissue. - View Dependent Claims (16, 17)
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18. A non-transitory computer-readable storage medium comprising instructions which, responsive to being executed by a processor, cause the processor to perform operations comprising:
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obtaining digitized optical images of prostate tissue reference samples that are Hematoxylin and Eosin (HE) stained; obtaining corresponding digitized infrared (IR) images of the prostate tissue reference samples; registering the digitized optical images with the corresponding digitized IR images, thereby forming composite images; segmenting the composite images into epithelium nuclei and lumen; extracting a plurality of morphologic feature information from the segmented composite images; storing the extracted plurality of morphologic feature information in a database; generating a proper subset of morphologic features from the plurality of morphologic features using a minimum-redundancy-maximal relevance criterion resulting in best performance; and modifying the proper subset of morphologic features using a sequential floating forward selection method to add morphologic features from the plurality of morphologic features that are not in the proper subset of morphologic features that provide a greatest increase performance. - View Dependent Claims (19)
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Specification