Algal elongase 6
First Claim
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1. A transformation vector comprising a polynucleotide sequence encoding a polypeptide having elongase 6 activity and comprises the amino acid sequence of SEQ ID NO:
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Abstract
Provided herein are exemplary isolated nucleotide sequences encoding polypeptides having elongase activity, which utilize fatty acids as substrates.
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8 Claims
- 1. A transformation vector comprising a polynucleotide sequence encoding a polypeptide having elongase 6 activity and comprises the amino acid sequence of SEQ ID NO:
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5. A transformed algal cell with increased or decreased poly unsaturated fatty acid biosynthesis, wherein said transformed algal cell has, respectively, enhanced or suppressed expression level of elongase 6 comprising the amino acid sequence of SEQ ID NO:
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wherein said enhanced expression comprises replacement of an endogenous promoter with a strong promoter in front of a gene encoding said elongase 6 comprising the amino acid sequence of SEQ ID NO;
14,wherein said suppressed expression is selected from the group consisting of replacement of an endogenous promoter with a weak promoter in front of a gene encoding said elongase 6, an insertion in a gene encoding said elongase 6, and a deletion or a substitution of a portion or the full length of a gene encoding said elongase 6 comprising the amino acid sequence of SEQ ID NO;
14,wherein said strong promoter is stronger than said endogenous promoter and said weak promoter is weaker than said endogenous promoter. - View Dependent Claims (6, 7, 8)
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Specification