Algal elongase 6

• US 9,783,812 B2
• Filed: 10/12/2015
• Issued: 10/10/2017
• Est. Priority Date: 06/08/2009
• Status: Active Grant

• Current Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Original Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Inventors: Kilian, Oliver, Vick, Bertrand
• Primary Examiner(s): Holland, Paul J
• Application Number:
  US14/880,979
• Publication Number:
  US 20160053273A1
• Time in Patent Office: 729 Days
• Field of Search: None
• CPC Class Codes: C12N 15/79 Vectors or expression systems specially adapted for eukaryotic hosts
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/1029 transferring groups other than amino-acyl groups (2.3.1)
• CPC Class Codes: C12N 9/93 Ligases (6)
• CPC Class Codes: C12Y 602/01003 Long-chain-fatty-acid-CoA ligase (6.2.1.3)

Abstract

Provided herein are exemplary isolated nucleotide sequences encoding polypeptides having elongase activity, which utilize fatty acids as substrates.

Claims

1. A transformation vector comprising a polynucleotide sequence encoding a polypeptide having elongase 6 activity and comprises the amino acid sequence of SEQ ID NO:

14.

2. The transformation vector of claim 1, wherein the said polynucleotide sequence comprises the nucleotide sequence of SEQ ID NO:

6.

3. The transformation vector of claim 1, further comprising a Violaxanthin-chlorophyll a binding protein (VCP) promoter.

4. The transformation vector of claim 1, wherein said transformation vector has been incorporated into the genome of an algal cell.

5. A transformed algal cell with increased or decreased poly unsaturated fatty acid biosynthesis, wherein said transformed algal cell has, respectively, enhanced or suppressed expression level of elongase 6 comprising the amino acid sequence of SEQ ID NO:

14,

wherein said enhanced expression comprises replacement of an endogenous promoter with a strong promoter in front of a gene encoding said elongase 6 comprising the amino acid sequence of SEQ ID NO;

14,

wherein said suppressed expression is selected from the group consisting of replacement of an endogenous promoter with a weak promoter in front of a gene encoding said elongase 6, an insertion in a gene encoding said elongase 6, and a deletion or a substitution of a portion or the full length of a gene encoding said elongase 6 comprising the amino acid sequence of SEQ ID NO;

14,

wherein said strong promoter is stronger than said endogenous promoter and said weak promoter is weaker than said endogenous promoter.

6. The transformed algal cell of claim 5, wherein said transformed algal cell comprises a knock-out mutant of said elongase 6.

7. The transformed algal cell of claim 5, wherein said transformed algal cell is a Nannochloropsis cell.

8. The transformed algal cell of claim 7, wherein said Nannochloropsis cell is Nannochloropsis oceanica.