Method and system for microfluidic particle orientation and/or sorting
First Claim
1. A system for sorting particles in a microfluidic system comprising:
- at least one channel suitable for fluid flow within the channel comprising at least one input source and at least two output sources, and a plurality of stages arranged along the channel;
an orientation stage following, and in fluid communication with, the at least one input source, the orientation stage comprising at least one optical waveguide arranged and configured to expose particles to radiation pressure to cause at least a majority of the particles to adopt a particular orientation in the fluid flow;
a detection stage following, and in fluid communication with, the orientation stage, the detection stage comprising an optical detector configured to detect at least one difference or discriminate between particles in the fluid flow past the detection stage, anda switching stage comprising a plurality of optical waveguides arranged such that particles flow past each optical waveguide in the plurality, said optical waveguides arranged and configured to expose particles to radiation pressure to cause a change in direction of movement of selected particles in the fluid flow to sort the particles into one of the at least two output sources based on input from the detection stage, and a controller.
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Accused Products
Abstract
A system for orienting particles in a microfluidic system includes one or more radiation pressure sources arranged to expose particles to radiation pressure to cause the particles to adopt a particular orientation in the fluid. A system for sorting particles in a microfluidic system includes a detection stage arranged to detect at least one difference or discriminate between particles in the fluid flow past the detection stage, and one or more radiation pressure sources past which the particles move sequentially and a controller arranged to switch radiation energy to cause a change in direction of movement of selected particles in the fluid flow to sort the particles. The particles may be biological particles such as spermatazoa. The radiation pressure may be optical pressure and may be from one or more waveguides which may extend across a channel of the microfluidic system.
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Citations
15 Claims
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1. A system for sorting particles in a microfluidic system comprising:
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at least one channel suitable for fluid flow within the channel comprising at least one input source and at least two output sources, and a plurality of stages arranged along the channel; an orientation stage following, and in fluid communication with, the at least one input source, the orientation stage comprising at least one optical waveguide arranged and configured to expose particles to radiation pressure to cause at least a majority of the particles to adopt a particular orientation in the fluid flow; a detection stage following, and in fluid communication with, the orientation stage, the detection stage comprising an optical detector configured to detect at least one difference or discriminate between particles in the fluid flow past the detection stage, and a switching stage comprising a plurality of optical waveguides arranged such that particles flow past each optical waveguide in the plurality, said optical waveguides arranged and configured to expose particles to radiation pressure to cause a change in direction of movement of selected particles in the fluid flow to sort the particles into one of the at least two output sources based on input from the detection stage, and a controller. - View Dependent Claims (2, 3, 4, 5, 6, 7, 11, 12, 13, 14, 15)
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8. A method of sorting particles in a microfluidic system, comprising,
forcing particles to adopt a particular orientation in fluid flow within at least one channel of the microfluidic system by contacting the particles with a radiation force produced by at least one optical waveguide; -
following orientation, detecting at least one difference, or discriminating between, particles in the fluid flow; and causing a change in the direction of movement of selected particles in the fluid flow to sort the particles based on the at least one difference or discrimination detected, wherein the change in direction is caused by contacting the particles with a radiation force produced by a plurality of optical waveguides. - View Dependent Claims (9, 10)
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Specification