Alkaline activation for immobilization of DNA taggants

• US 9,790,538 B2
• Filed: 03/07/2013
• Issued: 10/17/2017
• Est. Priority Date: 03/07/2013
• Status: Active Grant

• Current Assignee: APDN (B.V.I.) Inc. (Applied DNA Sciences Incorporated)
• Original Assignee: APDN (B.V.I.) Inc. (Applied DNA Sciences Incorporated)
• Inventors: Berrada, Abdelkrim, Liang, MingHwa Benjamin, Jung, Lawrence, Jensen, Kurt
• Primary Examiner(s): Crow, Robert T
• Application Number:
  US13/789,093
• Publication Number:
  US 20140256881A1
• Time in Patent Office: 1,685 Days
• Field of Search: None
• CPC Class Codes: B42D 25/21 for multiple purposes
• CPC Class Codes: B42D 25/382 absorbing or reflecting infrared light
• CPC Class Codes: B42D 25/387 absorbing or reflecting ultraviolet light
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 2563/185 Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals

Abstract

The invention provides methods for stably binding and immobilizing deoxyribonucleic acid onto objects and substrates. The method includes exposing the deoxyribonucleic acid to alkaline conditions, and contacting the deoxyribonucleic acid to the object or substrate. The alkaline conditions are produced by mixing the deoxyribonucleic acid with an alkaline solution having a pH of about 9.0 or higher, and contacting the deoxyribonucleic acid to the substrate. The immobilized DNA can be used as a taggant and can be used in combination with other detectable taggants, such as optical reporters. Methods for authentication of a DNA marked object are also provided.

Claims

1. A method of binding a deoxyribonucleic acid to the surface of a non-metal substrate which is not nylon or plastic, the method comprising:

providing a deoxyribonucleic acid;

exposing the deoxyribonucleic acid to an alkaline metal hydroxide solution comprising an alkali metal hydroxide at a concentration in a range from about 0.1M to about 1.0 M and incubating the mixture for from about 1 minute to about 6 hours at a temperature of from about 15°

C. to about 25°

C. to produce an alkaline-treated deoxyribonucleic acid, wherein the alkaline metal hydroxide solution is selected from the group consisting of lithium hydroxide, sodium hydroxide and cesium hydroxide; and

contacting the alkaline treated deoxyribonucleic acid to the surface of the non-metal substrate, for a period of time sufficient for the alkaline-treated deoxyribonucleic acid to covalently bind to the surface of the non-metal substrate.

3. The method of claim 1, wherein the non-metal substrate is selected from the group consisting of cotton, wool, glass, wood, printing ink, and a pharmaceutical composition.

2. A method of binding a deoxyribonucleic acid to the surface of a non-metal substrate which is not nylon or plastic, the method comprising:

providing a deoxyribonucleic acid;

exposing the deoxyribonucleic acid to an alkaline metal hydroxide solution comprising an alkali metal hydroxide at a concentration of about 0.6M and incubating the mixture for from about 10 minutes to about 2 hours at a temperature of from about 18°

C. to about 22°

C. to produce an alkaline-treated deoxyribonucleic acid, wherein the alkaline metal hydroxide solution is sodium hydroxide; and

contacting the alkaline treated deoxyribonucleic acid to the surface of the non-metal substrate, for a period of time sufficient for the alkaline-treated deoxyribonucleic acid to covalently bind to the surface of the non-metal substrate.

4. The method of claim 2, wherein the non-metal substrate is selected from the group consisting of cotton, wool, glass, wood, printing ink, and a pharmaceutical composition.