Method for measuring ATR inhibition mediated increases in DNA damage
First Claim
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1. A method for monitoring DNA damage in a subject by measuring accumulation of γ
- H2AX in blood, the method comprising;
a) treating a subject with an ATR inhibitor;
b) taking blood samples from the subject at various intervals;
c) treating the blood samples with a DNA damaging agent;
d) isolating white blood cells from the blood samples; and
e) measuring γ
H2AX levels in the white blood cells by using antibodies specific for γ
H2AX, wherein the ATR inhibitor is;
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Abstract
The present relates to methods for detecting DNA damage in subjects treated with an ATR inhibitor. More specifically, this invention relates to a method for measuring changes in levels of γH2AX and/or pChk1Ser345 in, e.g., surrogate tissue cells, following ex vivo stimulation with a DNA damaging agent.
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Citations
17 Claims
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1. A method for monitoring DNA damage in a subject by measuring accumulation of γ
- H2AX in blood, the method comprising;
a) treating a subject with an ATR inhibitor; b) taking blood samples from the subject at various intervals; c) treating the blood samples with a DNA damaging agent; d) isolating white blood cells from the blood samples; and e) measuring γ
H2AX levels in the white blood cells by using antibodies specific for γ
H2AX, wherein the ATR inhibitor is; - View Dependent Claims (2, 3, 4, 5, 6)
- H2AX in blood, the method comprising;
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7. A method for monitoring DNA damage in a subject by measuring changes in γ
- H2AX and/or pChk1Ser345, the method comprising;
administering an ATR inhibitor to a subject; taking surrogate tissue cell samples from the subject at various intervals; treating the surrogate tissue cell samples with a DNA damaging agent; measuring γ
H2AX and/or pChk1Ser345 levels in the surrogate tissue cell samples using antibodies specific for γ
H2AX and/or pChk1Ser345. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
- H2AX and/or pChk1Ser345, the method comprising;
Specification