Ratiometric immunoassay method and blood testing device
First Claim
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1. A cartridge for performing a ratiometric assay in a sample, comprising:
- a sample holding chamber oriented between a sample entry port and a sample extraction unit, wherein a portion of said sample extraction unit is configured to define a metered volume of a sample;
an analysis conduit in fluid communication with the sample extraction unit, the analysis conduit including a sensing region comprising a first sensor comprising an immunosensor for a first analyte, and a second sensor comprising an immunosensor for a second analyte;
a diluent conduit in fluid communication with the sample extraction unit, wherein said diluent conduit is oriented between a diluent entry port and a diluent introduction port, the diluent conduit includes an air introduction port disposed between the diluent entry port and the diluent introduction port, a volume within the diluent conduit between the air introduction port and the diluent introduction port defines a metered volume of a diluent, and the diluent introduction port is configured to dispel the metered volume of the diluent over and/or through the sample extraction unit to create a diluted sample;
a diluent chamber in fluid communication with the diluent conduit and containing the diluent; and
a pump configured to transfer at least a portion of the diluent through said diluent conduit, over and/or through said sample extraction unit, and into said analysis conduit.
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Abstract
The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field.
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19 Claims
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1. A cartridge for performing a ratiometric assay in a sample, comprising:
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a sample holding chamber oriented between a sample entry port and a sample extraction unit, wherein a portion of said sample extraction unit is configured to define a metered volume of a sample; an analysis conduit in fluid communication with the sample extraction unit, the analysis conduit including a sensing region comprising a first sensor comprising an immunosensor for a first analyte, and a second sensor comprising an immunosensor for a second analyte; a diluent conduit in fluid communication with the sample extraction unit, wherein said diluent conduit is oriented between a diluent entry port and a diluent introduction port, the diluent conduit includes an air introduction port disposed between the diluent entry port and the diluent introduction port, a volume within the diluent conduit between the air introduction port and the diluent introduction port defines a metered volume of a diluent, and the diluent introduction port is configured to dispel the metered volume of the diluent over and/or through the sample extraction unit to create a diluted sample; a diluent chamber in fluid communication with the diluent conduit and containing the diluent; and a pump configured to transfer at least a portion of the diluent through said diluent conduit, over and/or through said sample extraction unit, and into said analysis conduit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification