Oligonucleotide compositions with enhanced efficiency
First Claim
1. A composition comprising a combination of double-stranded oligonucleotides, the combination consisting of three or four different double-stranded oligonucleotides,wherein each double-stranded oligonucleotide is targeted to a different sequence within a single target gene,each double-stranded oligonucleotide consists of two separate strands,each strand is between 20 and 30 nucleomonomers in length,wherein the combination is capable of RNA interference (RNAi), andwherein at least one of the oligonucleotides comprises at least one modified internucleoside linkage orwherein at least one of the oligonucleotides comprises at least one modified sugar moiety.
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Abstract
The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. This invention also provides methods of inhibiting protein synthesis in a cell and methods of identifying oligonucleotide compositions that inhibit synthesis of a protein in a cell.
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Citations
20 Claims
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1. A composition comprising a combination of double-stranded oligonucleotides, the combination consisting of three or four different double-stranded oligonucleotides,
wherein each double-stranded oligonucleotide is targeted to a different sequence within a single target gene, each double-stranded oligonucleotide consists of two separate strands, each strand is between 20 and 30 nucleomonomers in length, wherein the combination is capable of RNA interference (RNAi), and wherein at least one of the oligonucleotides comprises at least one modified internucleoside linkage or wherein at least one of the oligonucleotides comprises at least one modified sugar moiety.
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10. A composition comprising a combination of double-stranded oligonucleotides, the combination consisting of two different double-stranded oligonucleotides,
wherein each double-stranded oligonucleotide is targeted to a different sequence within a single target gene, each double-stranded oligonucleotide consists of two separate strands, each strand is between 20 and 30 nucleomonomers in length, wherein the combination is capable of RNA interference (RNAi), and wherein at least one of the oligonucleotides comprises at least one modified internucleoside linkage or wherein at least one of the oligonucleotides comprises at least one modified sugar moiety.
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19. A composition comprising a combination of double-stranded oligonucleotides, the combination consisting of five, six, seven or eight different double-stranded oligonucleotides,
wherein each double-stranded oligonucleotide is targeted to a different sequence within a single target gene, each double-stranded oligonucleotide consists of two separate strands, each strand is between 20 and 30 nucleomonomers in length, wherein the combination is capable of RNA interference (RNAi), and wherein at least one of the oligonucleotides comprises at least one modified internucleoside linkage or wherein at least one of the oligonucleotides comprises at least one modified sugar moiety.
Specification