Nucleic acid sequence measuring method, nucleic acid sequence measuring device, manufacturing method for nucleic acid sequence measuring device, and nucleic acid sequence measuring apparatus

• US 9,797,003 B2
• Filed: 08/26/2014
• Issued: 10/24/2017
• Est. Priority Date: 08/27/2013
• Status: Active Grant

• Current Assignee: Yokogawa Electric Corporation
• Original Assignee: Yokogawa Electric Corporation
• Inventors: Tadenuma, Takashi, Taguchi, Tomoyuki, Tanaami, Takeo
• Primary Examiner(s): Whisenant, Ethan C
• Application Number:
  US14/469,563
• Publication Number:
  US 20150065377A1
• Time in Patent Office: 1,155 Days
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2537/1373 Displacement by a nucleic acid
• CPC Class Codes: C12Q 2537/161 A competitive reaction step
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

A nucleic acid sequence measuring method includes measuring fluorescence from the nucleic acid sequence measuring device supplied with a sample solution. The device includes a fluorescent probe added with a fluorescent molecule, and a quenching probe added with a quenching substance. The fluorescent probe and/or the quenching probe has a detection part detecting a predetermined nucleic acid sequence. Fluorescence from the fluorescent molecule is quenched by the quenching substance coupled with the fluorescent molecule when the hybridization between the detection target nucleic acid and the detection part has not occurred, and fluorescence is emitted from the fluorescent molecule separated from the quenching substance when the hybridization has occurred.

Claims

1. A nucleic acid sequence measuring method comprising:

preparing a sample solution containing a target nucleic acid;

supplying the sample solution to a nucleic acid sequence measuring device; and

measuring fluorescence from the nucleic acid sequence measuring device, the nucleic acid sequence measuring device including;

a fluorescent probe having a coupling part and a base end and added with a fluorescent molecule at a predetermined position;

a quenching probe having a coupling part and a base end and added with a quenching substance at a predetermined position; and

a substrate having a solid surface to which the base end of each of the fluorescent probe and the quenching probe is fixed, wherein;

the coupling part of the fluorescent probe and the coupling part of the quenching probe each have a mutually complementary nucleic acid sequence;

at least one of the fluorescent probe and the quenching probe has a detection part with a nucleic acid sequence complementary with a particular nucleic acid sequence; and

the base end of the fluorescent probe and the base end of the quenching probe are fixed to the solid surface, the probes are in such a positional relationship that fluorescence from the fluorescent molecule is quenched by the quenching substance that has approached the fluorescent molecule by maintaining the coupling between the fluorescent probe and the quenching probe through the coupling parts when the hybridization between the target nucleic acid and the detection part has not occurred, and that fluorescence is emitted from the fluorescent molecule separated from the quenching substance due to canceling of the coupling between the fluorescent probe and the quenching probe through the coupling parts when the hybridization between the target nucleic acid and the detection part has occurred.

2. The nucleic acid sequence measuring method according to claim 1, wherein the fluorescence from the nucleic acid sequence measuring device is measured in the presence of the sample solution.

3. A nucleic acid sequence measuring device configured to measure a predetermined nucleic acid sequence of a target nucleic acid in a sample solution, comprising:

a fluorescent probe having a coupling part and a base end and added with a fluorescent molecule at a predetermined position;

a quenching probe having a coupling part and a base end and added with a quenching substance at a predetermined position; and

a substrate having a solid surface to which the base end of each of the fluorescent probe and the quenching probe is fixed, wherein;

the coupling part of the fluorescent probe and the coupling part of the quenching probe each have a mutually complementary nucleic acid sequence;

at least one of the fluorescent probe and the quenching probe has a detection part with a nucleic acid sequence complementary with a particular nucleic acid sequence; and

the base end of the fluorescent probe and the base end of the quenching probe are fixed to the solid surface, the probes are in such a positional relationship that fluorescence from the fluorescent molecule is quenched by the quenching substance that has approached the fluorescent molecule by maintaining the coupling between the fluorescent probe and the quenching probe through the coupling parts when the hybridization between the target nucleic acid and the detection part has not occurred, and that fluorescence is emitted from the fluorescent molecule separated from the quenching substance due to canceling of the coupling between the fluorescent probe and the quenching probe through the coupling parts when the hybridization between the target nucleic acid and the detection part has occurred.

4. The nucleic acid sequence measuring device according to claim 3, wherein the fluorescent probe has the detection part.

5. The nucleic acid sequence measuring device according to claim 3, wherein the substrate is a flat plate and the solid surface is one plane of the flat plate.

6. The nucleic acid sequence measuring device according to claim 3, wherein the substrate is a sphere and the solid surface is a spherical surface of the sphere.

7. The nucleic acid sequence measuring device according to claim 3, wherein at least a part of the coupling part functions as the detection part.

8. The nucleic acid sequence measuring device according to claim 3, wherein the number of quenching probes is larger than that of fluorescent probes.

9. The nucleic acid sequence measuring device according to claim 8, wherein the ratio between the number of fluorescent probes and the number of quenching probes is 1:

3.

10. The nucleic acid sequence measuring device according to claim 3, wherein the predetermined position at which the fluorescent molecule and the quenching substance are added is in the middle of the fluorescent probe and the quenching probe.

11. The nucleic acid sequence measuring device according to claim 3, wherein there is a plurality of predetermined positions at which the fluorescent molecule and the quenching substance are added.

12. The nucleic acid sequence measuring device according to claim 3, wherein both the fluorescent probe and the quenching probe have the detection part.

13. A manufacturing method for the nucleic acid sequence measuring device according to claim 3, comprising:

coupling the fluorescent probe and the quenching probe through the coupling parts; and

coupling the fluorescent probe and the quenching probe to the solid surface with the probes coupled with each other through the coupling parts.

14. A nucleic acid sequence measuring apparatus comprising:

the nucleic acid sequence measuring device according to claim 3; and

a fluorescence reading apparatus configured to measure the fluorescence from the nucleic acid sequence measuring device.

15. The nucleic acid sequence measuring apparatus according to claim 14, wherein the fluorescence from the nucleic acid sequence measuring device is measured in the presence of the sample solution.

16. The nucleic acid sequence measuring device according to claim 3, wherein the fluorescent molecule is positioned distally from the base end of the fluorescent probe, and the quenching substance is positioned distally from the base end of the quenching probe.