Multi-mode separation for target detection
First Claim
1. A method of forming, manipulating and isolating a sandwich complex, wherein the complex consists of one or more magnetic beads, one or more buoyant beads, and a target analyte, and where the one or more buoyant beads in the complex provide sufficient buoyancy such that the complex floats, the method comprising:
- (a) contacting the sample in a solution with (i) a population of the one or more magnetic beads, each magnetic bead comprising a moiety that can specifically associate with the target analyte under appropriate conditions and (ii) a population of the one or more buoyant beads, each buoyant bead comprising a moiety that can specifically associate with the target analyte under appropriate conditions, wherein contact results in formation of the complex containing the one or more magnetic beads, the one or more buoyant beads, and the target analyte, such that the complex is both magnetic and buoyant; and
(b) isolating the complex based on the combined movement of the complex in a magnetic field and in a gravitational or centrifugal field.
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Accused Products
Abstract
Sandwich separation is based on forming a sandwich complex with a magnetic bead, buoyant bead, and a target. Once a sandwich formation is created, the sandwich can be separated using its dual physical properties, namely magnetism and buoyancy. Sandwich separation is highly specific, allows for removal of the beads that do not have any attached target, and reduces the number of background beads. Sandwich separation can also be used to allow for target detection in raw specimen. Also, improvement of detection capability is accomplished by performing AMBR measurements on a solid interface, where the rotational period speeds up and allows for dramatically reduced time-to-result.
82 Citations
17 Claims
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1. A method of forming, manipulating and isolating a sandwich complex, wherein the complex consists of one or more magnetic beads, one or more buoyant beads, and a target analyte, and where the one or more buoyant beads in the complex provide sufficient buoyancy such that the complex floats, the method comprising:
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(a) contacting the sample in a solution with (i) a population of the one or more magnetic beads, each magnetic bead comprising a moiety that can specifically associate with the target analyte under appropriate conditions and (ii) a population of the one or more buoyant beads, each buoyant bead comprising a moiety that can specifically associate with the target analyte under appropriate conditions, wherein contact results in formation of the complex containing the one or more magnetic beads, the one or more buoyant beads, and the target analyte, such that the complex is both magnetic and buoyant; and (b) isolating the complex based on the combined movement of the complex in a magnetic field and in a gravitational or centrifugal field. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification