Substrates, devices, and methods for quantitative liquid crystal assays

• US 9,797,843 B2
• Filed: 07/28/2015
• Issued: 10/24/2017
• Est. Priority Date: 08/27/2001
• Status: Active Grant

• Current Assignee: Platypus Technologies, LLC
• Original Assignee: Platypus Technologies, LLC
• Inventors: Abbott, Nicholas, Murphy, Christopher, Israel, Barbara
• Primary Examiner(s): LAM, ANN Y
• Application Number:
  US14/811,083
• Publication Number:
  US 20160025636A1
• Time in Patent Office: 819 Days
• Field of Search: None
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 21/75 Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated systems in which material is burnt in a flame or plasma G01N21/72, G01N21/73
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal assay format.

Claims

1. A system for detecting an analyte in a sample comprising:

a. at least one substrate having a surface comprising recognition moieties;

b. a mesogenic layer oriented on said surface;

c. electrodes positioned to apply an electrical field across said surface comprising recognition moieties.

2. The system of claim 1, wherein said system further comprises an interface between said mesogenic layer and a member selected from the group consisting of gases, liquids, solids, and combinations thereof.

3. The system of claim 1, wherein said recognition moiety is attached to said surface by an interaction which is a member selected from the group consisting of covalent bonding, ionic bonding, chemisorption, physisorption, and combinations thereof.

4. The system of claim 1, wherein said surface further comprises an organic layer.

5. The system of claim 4, wherein said recognition moiety is attached to said organic layer by an interaction which is a member selected from the group consisting of covalent bonding, ionic bonding, chemisorption, physisorption, and combinations thereof.

6. The system of claim 1, wherein said mesogenic layer comprises a polymeric mesogen.

7. The system of claim 1, wherein said mesogenic layer comprises mesogens selected from the group consisting of 4 cyano 4′

pentylbiphenyl, N (4-methoxybenzylidene) 4 butlyaniline and combinations thereof.

8. The system of claim 1, wherein said mesogenic layer comprises a lyotropic liquid crystal.

9. The system of claim 1, wherein said surface is a metal surface.

10. The system of claim 9, wherein said metal surface is selected from the group consisting of gold, platinum, palladium, copper, nickel, silver, and combinations thereof.

11. The system of claim 1, wherein said substrate is selected from the group consisting of flexible substrates, rigid substrates, optically opaque substrates, optically transparent substrates, conducting substrates, semiconducting substrates, and combinations thereof.

12. The system of claim 1, wherein said substrate is selected from the group consisting of inorganic crystals, inorganic glasses, inorganic oxides, metals, organic polymers, and combinations thereof.

13. The system of claim 12, wherein said organic polymer is selected from the group consisting of polyvinylidene fluoride, polydimethylsiloxane, polycarbonate, polystyrene, polyurethane, polyisocyanoacrylate, epoxy and combinations thereof.

14. The system of claim 1, wherein said analyte is selected from the group consisting of polypeptides, polynucleotides, organic analytes, and pathogens.

15. The system of claim 1, wherein said recognition moiety is selected from the group consisting of polynucleotides, antigen binding molecules, and polypeptides.