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Biosensor, measuring instrument for biosensor, and method of quantifying substrate

  • US 9,797,858 B2
  • Filed: 01/29/2014
  • Issued: 10/24/2017
  • Est. Priority Date: 11/30/2000
  • Status: Expired due to Term
First Claim
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1. A method of measuring a quantity of a substrate in a sample liquid by inserting a biosensor into a measuring device, the method comprising:

  • providing the biosensor which includes;

    an insulating board;

    an electrode part which includes a counter electrode and a measuring electrode disposed on at least a part of the insulating board;

    a sample supplying path which supplies the sample liquid to the electrode part; and

    a reagent layer at least partially disposed in the sample supplying path;

    providing the measuring device which includes;

    a supporting section which detachably supports the biosensor;

    a connecting terminal which applies a voltage to the electrode part; and

    a driving power supply;

    applying the voltage to the electrode part by the driving power supply when the biosensor is inserted into the supporting section;

    detecting a start of supplying the sample liquid to the sample supplying path by detecting that a first output signal from the electrode part reaches a given threshold;

    determining that a sufficient quantity of the sample liquid is supplied to the sample supplying path by detecting that a second output signal from the electrode part reaches a given threshold within a predetermined period after the start of supplying the sample liquid to the sample supplying path;

    measuring an elapsed time between the first output signal and the second output signal;

    measuring the quantity of the substrate of the sample liquid based on a third output signal from the electrode part; and

    if the elapsed time is within a predetermined time, correcting the measurement of the quantity of the substrate of the sample liquid based on the elapsed time, orif the elapsed time is not within a predetermined time, terminating the measurement of the quantity of the substrate of the sample liquid.

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