Slip-induced compartmentalization

• US 9,803,237 B2
• Filed: 04/24/2013
• Issued: 10/31/2017
• Est. Priority Date: 04/24/2012
• Status: Active Grant

• Current Assignee: Talis Biomedical Corporation (Baker Bros. Advisors LP), California Institute of Technology
• Original Assignee: California Institute of Technology, Slipchip Corporation
• Inventors: Ismagilov, Rustem F., Shen, Feng
• Primary Examiner(s): Siefke, Sam P
• Application Number:
  US13/869,856
• Publication Number:
  US 20130281316A1
• Time in Patent Office: 1,651 Days
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/161 Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/065 sliding valves
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]

Abstract

The present invention relates to fluidic devices for compartmentalizing samples. In particular, the devices and related systems and methods allow for compartmentalization by using one or more first chambers connect by a first channel (e.g., where the cross-sectional dimension of the first channel is less than the cross-sectional dimension of at least one first chamber).

Claims

1. A system for compartmentalization comprising a device, a target fluid, an immiscible fluid, and a surface energy,

wherein the device comprises

(i) a first layer comprising a first region;

and

(ii) a second layer comprising a plurality of second regions;

wherein said first region and said plurality of second regions are connected by relative movement of the first and second layers from a first position, wherein the first region is not connected to the plurality of second regions, to a second position, wherein the first region is connected to the plurality of second regions to form a plurality of combined regions,

wherein, in said first position, said target fluid is present in said first region, and wherein relative movement of said device from said first position to said second position results in separation of said target fluid into compartments that are separated by said immiscible fluid and located in said plurality of combined regions, and

wherein, in said second position, the surface energy of the system is reduced from a first surface energy before said separation of said target fluid into said compartments to a second surface energy after said separation of said target fluid into said compartments.

2. The system of claim 1, wherein said device is a microfluidic device.

3. The system of claim 1, wherein said first region comprises a first channel and wherein said plurality of second regions comprises a plurality of chambers, a plurality of hydrophilic surfaces, or a plurality of hydrophobic surfaces.

4. The system of claim 3, further comprising an array of said first channels and/or an array of said plurality of second regions.

5. The system of claim 3, wherein said second layer comprises said plurality of chambers.

6. The system of claim 3, wherein said plurality of second regions comprises said plurality of hydrophilic surfaces, wherein said plurality of hydrophilic surfaces alternate with and are fluidically connected to a plurality of hydrophobic surfaces.

7. The system of claim 1, wherein said first region is one of a plurality of fluidically connected first regions, and said plurality of second regions comprises a plurality of chambers, a plurality of hydrophilic surfaces, or a plurality of hydrophobic surfaces.

8. The system of claim 7, further comprising an array of said plurality of first regions and/or an array of said plurality of second regions.

9. The system of claim 7, wherein said plurality of first regions comprises said plurality of hydrophilic surfaces, and said plurality of second regions comprises a plurality of hydrophilic surfaces, wherein the hydrophilic surfaces of the plurality of first and second regions alternate with hydrophobic surfaces.

10. The system of claim 7, wherein said plurality of first regions comprises said plurality of hydrophobic surfaces, and said plurality of second regions comprises a plurality of hydrophobic surfaces, wherein the hydrophobic surfaces of the plurality of first and second regions alternate with hydrophilic surfaces.

11. The system of claim 7, wherein said first layer further comprises a plurality of third regions alternating with said plurality of first regions.

12. The system of claim 11, wherein said plurality of first regions comprises chambers, and said plurality of third regions comprises channels connecting said chambers, wherein a cross-sectional dimension of said channel is less than a cross-sectional dimension of said chambers.

13. The system of claim 12, wherein said plurality of second regions comprises chambers.

14. The system of claim 13, wherein the second layer further comprises a plurality of fourth regions, which are chambers alternating with and not fluidically connect to the plurality of second regions, wherein the third and fourth regions are connected by the relative movement.

15. The system of claim 12, wherein said plurality of second regions comprises hydrophilic or hydrophobic surfaces, and said second layer comprises alternating hydrophobic and hydrophilic surfaces.

16. The system of claim 1, wherein said second layer further comprises a plurality of fifth regions that are not fluidically connected with the plurality of second regions, wherein a second relative movement of the first or second layers connects the compartments with the fifth regions.

17. The system of claim 16, wherein the fifth regions are fluidically connected.

18. The system of claim 17, wherein the fifth regions contain a reagent fluid.

19. The system of claim 17, wherein the second relative movement results in separation of said reagent fluid and mixture with said compartments.

20. The system of claim 17, wherein the first region is connected to one of the plurality of second regions and one of the plurality of fifth regions after the second relative movement.

21. The system of claim 1, wherein the first layer comprises a sixth region that is not fluidically connected to the first region, and the compartments are located in the second layer and wherein a second relative movement of the first or second layer connects the compartments with the sixth region.

22. The system of claim 21, wherein the sixth region contains a reagent fluid.

23. The system of claim 22, wherein the second relative movement results in separation of said reagent fluid and mixture with said compartments.

24. The system of claim 1, wherein the first layer further comprises a first plurality of chambers that are not fluidically connected to each other or to the first region, and the second layer further comprises a second plurality of chambers that are not connected to each other or the plurality of second regions, wherein, prior to the relative movement, the first region is fluidically connected to the second plurality of chambers, the relative movement results in connecting of the first plurality of chambers to the second plurality of chambers to form a second plurality of combined regions, and results in further separation of said target fluid into second compartments that are separated by an immiscible fluid and located in said second plurality of combined regions.

25. The system of claim 1, wherein said immiscible fluid is a liquid lubricant.

26. The system of claim 25, wherein said target fluid comprises an aqueous fluid.

27. The system of claim 1, wherein said plurality of second regions contains said immiscible fluid.

28. The system of claim 1, wherein said first layer and/or second layer translates longitudinally to produce said relative movement.

29. The system of claim 1, wherein said first layer and/or second layer rotates axially on the same or different axis to produce said relative movement.

30. The system of claim 1, wherein the plurality of second regions comprises a binding agent.

31. The system of claim 30, wherein the binding agent is selected from the group consisting of an antibody, an antibody fragment, an oligopeptide, a polypeptide, a nucleic acid, a cellular receptor, a ligand, an aptamer, a MHC-peptide monomer or oligomer, biotin, avidin, an oligonucleotide, a coordination complex, a synthetic polymer, a carbohydrate, a charged surface, a modified nucleic acid, a nucleic acid analog, a filter, a matrix, a polymer, a charge switch material, a gel, a membrane, a fiber, a particle, a bead, an affinity resin, an ion exchange resin, a silica-based material, a magnetic material, and a combination thereof.

32. The system of claim 31, wherein at least two of the plurality of second regions have different binding agents.