Fast auto-focus in imaging

• US 9,813,608 B2
• Filed: 05/22/2015
• Issued: 11/07/2017
• Est. Priority Date: 02/01/2011
• Status: Active Grant

• Current Assignee: Roche Diagnostics Hematology Inc (Roche Holding AG)
• Original Assignee: Roche Diagnostics Hematology Inc (Roche Holding AG)
• Inventors: Zahniser, Michael
• Primary Examiner(s): WALKER, JARED T
• Application Number:
  US14/719,841
• Publication Number:
  US 20150256739A1
• Time in Patent Office: 900 Days
• Field of Search: 348 79
• CPC Class Codes: G02B 21/244 using image analysis techniques
• CPC Class Codes: G06T 2207/10148 Varying focus

Abstract

The disclosure relates to methods and systems for automatically focusing multiple images of one or more objects on a substrate. The methods include obtaining, by a processor, a representative focal distance for a first location on the substrate based on a set of focal distances at known locations on the substrate. The methods also include acquiring, by an image acquisition device, a set of at least two images of the first location. The images are each acquired using a different focal distance at an offset from the representative focal distance. The methods further include estimating, by a processor, an ideal focal distance corresponding to the first location based on comparing a quality of focus for each of the images, and storing the estimated ideal focal distance and the first location in the set of focal distances at known locations.

Claims

1. A method for automatically focusing multiple images of one or more objects on a substrate, the method comprising:

a) obtaining, by a processor, a representative focal distance for a first location on the substrate based on a set of focal distances at known locations on the substrate, wherein the representative focal distance is estimated as a weighted average of at least two focal distances from the set of focal distances at known locations, wherein a weight for a given focal distance is calculated based on a distance of the corresponding location from the first location;

b) acquiring, by an image acquisition device, a set of images of the first location based on the representative focal distance;

c) estimating, by a processor, an ideal focal distance corresponding to the first location based on the acquired set of images; and

d) storing the estimated ideal focal distance and the first location in the set of focal distances at known locations.

2. The method of claim 1, further comprising:

retrieving, by a processor, the set of focal distances at known locations;

acquiring, by the image acquisition device, an additional set of at least two images of a second location, wherein the images in the additional set are each acquired using a different focal distance at an offset from a second representative focal distance calculated for the second location based on the set of known focal distances at known locations; and

estimating, by a processor, an ideal focal distance corresponding to the second location based on comparing a quality of focus for each of the images in the additional set.

3. The method of claim 1, wherein each of the images in the acquired set have a corresponding focal distance at an offset from the representative focal distance.

4. The method of claim 1, wherein the ideal focal distance is estimated by comparing focus quality of images in the acquired set.

5. The method of claim 4, wherein comparing the quality of focus further comprises calculating, for each image, a focus score by quantifying differences between neighboring pixels.

6. The method of claim 5, wherein the focus score is a Brenner focus score.

7. The method of claim 5, wherein estimating the ideal focal distance further comprises:

calculating a difference between logarithms of the focus scores of the images;

estimating an offset from the calculated difference; and

estimating the ideal focal distance based on the offset.

8. The method of claim 1, wherein at least two of the images in the acquired set are acquired under illumination by a substantially same color of light, wherein the color of light is at least one of green, yellow, blue and red.

9. The method of claim 5, wherein estimating the ideal focal distance comprises fitting a curve representing logarithms of focus scores as a function of focal distance.

10. A system comprising:

an image acquisition device configured to acquire a set of images of a first location on a substrate based on a representative focal distance; and

one or more processing devices, connected to the image acquisition device, the one or more processing devices configured to;

compute the representative focal distance for the first location based on a set of focal distances at known locations on the substrate, wherein the representative focal distance is estimated as a weighted average of at least two focal distances from the set of focal distances at known locations, wherein a weight for a given focal distance is calculated based on a distance of the corresponding location from the first location,

estimate an ideal focal distance corresponding to the first location based on the acquired set of images, and

store the estimated ideal focal distance and the first location in the set of focal distances at known locations.

11. The system of claim 10, wherein the one or more processing devices are further configured to provide the computed representative focal distance to the image acquisition device.

12. The system of claim 10, wherein the set of images comprises at least two images, and is acquired using a different focal distance for each image at an offset from the representative focal distance.

13. The system of claim 10, wherein

the one or more processing devices are further configured to retrieve the set of focal distances at known locations;

and

the image acquisition device is further configured to acquire an additional set of at least two images of a second location, wherein the images in the additional set are each acquired using a different focal distance at an offset from a second representative focal distance calculated for the second location based on the set of known focal distances at known locations.

14. The system of claim 13 wherein the one or more processing devices are further configured to estimate an ideal focal distance corresponding to the second location based on comparing a quality of focus for each of the images in the additional set.

15. The system of claim 10, wherein the one or more processing devices are further configured to compare a quality of focus for the acquired set of images, wherein comparing the quality of focus further comprises calculating, for each image, a focus score by quantifying differences between neighboring pixels.

16. The system of claim 15, wherein the focus score is a Brenner focus score.

17. The system of claim 15, wherein the one or more processing devices are further configured to:

calculate a difference between logarithms of the focus scores of the images;

estimate an offset from the calculated difference; and

estimate the ideal focal distance based on the offset.

18. The system of claim 10, wherein at least two of the images in the acquired set are acquired under illumination by a substantially same color of light, wherein the color of light is at least one of green, yellow, blue and red.

19. The system of claim 15, wherein estimating the ideal focal distance comprises fitting a curve representing logarithms of focus scores as a function of focal distance.

20. A non-transitory computer readable storage device, having encoded thereon computer readable instructions that causes one or more processing devices to execute operations comprising:

obtaining a representative focal distance for a first location on a substrate based on a set of focal distances at known locations on the substrate, wherein the representative focal distance is estimated as a weighted average of at least two focal distances from the set of focal distances at known locations, wherein a weight for a given focal distance is calculated based on a distance of the corresponding location from the first location

estimating an ideal focal distance corresponding to the first location based on the acquired set of images,

storing the estimated ideal focal distance and the first location in the set of focal distances at known locations, and

providing the computed representative focal distance to an image acquisition device for the image acquisition device to acquire a set of images of the first location based on the representative focal distance.

21. The computer readable storage device of claim 20, wherein the operations further comprise:

comparing a quality of focus by calculating, for each image, a focus score by quantifying differences between neighboring pixels.

22. The computer readable storage device of claim 20, wherein the operations further comprise:

calculating a difference between logarithms of the focus scores of the images;

estimating an offset from the calculated difference; and

estimating the ideal focal distance based on the offset.