Polymers and DNA copolymer coatings

• US 9,815,916 B2
• Filed: 10/29/2015
• Issued: 11/14/2017
• Est. Priority Date: 10/31/2014
• Status: Active Grant

• Current Assignee: Illumina Cambridge Limited (Illumina Incorporated)
• Original Assignee: Illumina Cambridge Limited (Illumina Incorporated)
• Inventors: Brown, Andrew A., George, Wayne N., Richez, Alexandre, Dingwall, Anne-Cecile, von Hatten, Xavier
• Primary Examiner(s): JONES JR., ROBERT STOCKTON
• Application Number:
  US14/927,252
• Publication Number:
  US 20160122816A1
• Time in Patent Office: 747 Days
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C08F 20/60 containing nitrogen in addition to the carbonamido nitrogen
• CPC Class Codes: C08F 220/22 Esters containing halogen
• CPC Class Codes: C08F 220/282 and containing two or more oxygen atoms
• CPC Class Codes: C08F 220/325 containing glycidyl radical, e.g. glycidyl (meth)acrylate
• CPC Class Codes: C08F 220/346 and further oxygen
• CPC Class Codes: C08F 220/56 Acrylamide; Methacrylamide
• CPC Class Codes: C08F 220/60 containing nitrogen in addition to the carbonamido nitrogen
• CPC Class Codes: C08F 220/603 and containing oxygen in addition to the carbonamido oxygen and nitrogen
• CPC Class Codes: C08F 222/385 Monomers containing two or more (meth)acrylamide groups, e.g. N,N'-methylenebisacrylamide
• CPC Class Codes: C08F 2438/03 Use of a di- or tri-thiocarbonylthio compound, e.g. di- or tri-thioester, di- or tri-thiocarbamate, or a xanthate as chain transfer agent, e.g . Reversible Addition Fragmentation chain Transfer [RAFT] or Macromolecular Design via Interchange of Xanthates [MADIX]
• CPC Class Codes: C08F 285/00 Macromolecular compounds obtained by polymerising monomers on to preformed graft polymers
• CPC Class Codes: C08F 293/005 using free radical "living" or "controlled" polymerisation, e.g. using a complexing agent
• CPC Class Codes: C08F 8/32 by reaction with amines
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2565/401 Signal amplification by chemical polymerisation
• CPC Class Codes: C40B 50/18 using a particular method of attachment to the solid support

Abstract

Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.

Claims

1. A polymer for surface functionalization, comprising a recurring unit of Formula (I) and a recurring unit of Formula (II):

2. The polymer of claim 1, wherein R^1a is hydrogen or optionally substituted alkyl and each of R^2a and R^3a is hydrogen.

3. The polymer of claim 1, wherein R^1b is hydrogen or optionally substituted alkyl and each of R^2b and R^3b is hydrogen.

4. The polymer of claim 1, wherein L² is an optionally substituted alkylene.

5. The polymer of claim 1, wherein L¹ is an optionally substituted alkylene.

6. The polymer of claim 1, wherein the recurring unit of Formula (I) is represented by Formula (Ia) and Formula (II) is represented by Formula (IIa):

7. The polymer of claim 1, wherein L¹ is an optionally substituted heteroalkylene.

8. The polymer of claim 7, wherein L¹ is —

(CH₂)_m—

NH—

(CH₂)_n—

optionally substituted with one or more oxo, and wherein each m and n is independently an integer from 1 to 10.

9. The polymer of claim 1, wherein the recurring unit of Formula (I) is represented by Formula (Ib):

10. The polymer of claim 1, further comprising one or more recurring units selected from the group consisting of polyacrylamides, polyacrylates, polyurethanes, polysiloxanes, silicones, polyacroleins, polyphosphazenes, polyisocyanates, poly-ols, and polysaccharides, and combinations thereof.

11. The polymer of claim 10, further comprising one or more recurring units of Formula (IIIa) or (IIIb) or both:

12. The polymer of claim 11, wherein each R^4a, R^4b, and R^5b is independently hydrogen or methyl.

13. The polymer of claim 11, wherein R^6b and R^7b are both hydrogen.

14. The polymer of claim 11, wherein at least one of R^5a or R^6a is hydrogen.

15. The polymer of claim 11, wherein at least one of R^5a or R^6a is methyl.

16. The polymer of claim 11, wherein the one or more recurring units of Formula (IIIa) are represented by (IIIa1), (IIIa2), or (IIIa3):

17. The polymer of claim 11, wherein the one or more recurring units of Formula (IIIb) are represented by (IIIb1):

18. A substrate having a first surface comprising a polymer of claim 1 covalently bonded thereto.