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Multiplex amplification methods

  • US 9,816,134 B2
  • Filed: 08/11/2014
  • Issued: 11/14/2017
  • Est. Priority Date: 05/27/2010
  • Status: Active Grant
First Claim
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1. A method for amplifying an RNA target comprising:

  • (a) obtaining a capture probe comprising from the 5′

    end a second priming sequence, a tag sequence, a first priming sequence and a sequence complementary to the RNA target;

    (b) hybridizing the RNA target to the capture probe and extending the RNA using a polymerase and using the capture probe as template to form a duplex between the capture probe and the extended RNA target;

    (c) optionally destroying or removing the capture probe; and

    (d) amplifying the portion of the extended RNA comprising the first and second primer regions and the tag region.

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