Analyte detection system

• US 9,820,690 B1
• Filed: 07/16/2014
• Issued: 11/21/2017
• Est. Priority Date: 07/16/2014
• Status: Active Grant

• Current Assignee: Verily Life Sciences LLC (Alphabet Inc.)
• Original Assignee: Verily Life Sciences LLC (Alphabet Inc.)
• Inventors: Schwartz, Jerrod Joseph, Bajaj, Vikram Singh
• Primary Examiner(s): Winakur, Eric
• Application Number:
  US14/333,140
• Time in Patent Office: 1,224 Days
• CPC Class Codes: A61B 5/055 involving electronic [EMR] or nuclear [NMR] magnetic resonance, e.g. magnetic resonance imaging
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/1468 using chemical or electrochemical methods, e.g. by polarographic means A61B5/1486 takes precedence
• CPC Class Codes: A61B 5/4848 Monitoring or testing the effects of treatment, e.g. of medication
• CPC Class Codes: A61B 5/6802 Sensor mounted on worn items
• CPC Class Codes: A61B 5/681 Wristwatch-type devices

Abstract

A wearable device includes a mount to mount the wearable device on a living body and a detector to detect an analyte response signal transmitted from tissue in the living body. The tissue contains a biologically active agent in an inactive state and functionalized particles. The biologically active agent can be converted to an active state that can affect a biological state of the living body. The functionalized particles are configured to bind with a target analyte, the presence or absence or concentration or abundance of which is correlated with the biological state. The analyte response signal is related to interaction of the target analyte with the functionalized particles. A source can apply directed energy into the tissue that is sufficient to convert the biologically active agent from the inactive state to the active state. A processor can determine a presence or absence or concentration or abundance of the analyte based on the analyte response signal.

Claims

1. A system comprising:

a wearable device for detecting an analyte response signal and an unbound functionalized particle signal from tissue through an external surface of a living body, the wearable device comprising a mount adapted for mounting the wearable device on the external surface of a living body and a detector,

wherein the tissue contains a biologically active agent in an inactive state and functionalized particles, wherein the functionalized particles can interact with one or more target analytes, wherein the biologically active agent can be converted from the inactive state to an active state, wherein the concentration or abundance of the one or more target analytes is correlated with a biological state of the living body that is affected by the biologically active agent when in the active state, wherein the analyte response signal is related to interaction of the one or more target analytes with the functionalized particles, and wherein the unbound functionalized particle signal is related to functional particles that are not interacting with the one or more target analytes;

a directed energy source;

a modulation source;

a processor; and

a computer readable medium storing instructions executable by the processor to perform operations comprising;

controlling the directed energy source to apply directed energy into the tissue through the external surface, wherein the directed energy is sufficient to convert the biologically active agent from the inactive state to the active state;

controlling the detector to detect an analyte response signal transmitted from tissue through the external surface;

controlling the modulation source to modulate the analyte response signal and the unbound functionalized particle signal, such that the analyte response signal is affected differently than the unbound functionalized particle signal; and

controlling the processor to determine the concentration or abundance of the one or more target analytes by differentiating the analyte response signal from the unbound functionalized particle signal, based, at least in part, on the modulation.

2. The system of claim 1, wherein the biologically active agent comprises a pro-drug when in the inactive state and comprises a drug when in the active state.

3. The system of claim 1, wherein the biologically active agent is associated with a carrier when in the inactive state and is released from the carrier when in the active state.

4. The system of claim 3, wherein the carrier comprises a liposome, micelle, nanoparticle, nanocage or reservoir.

5. The system of claim 1, wherein the directed energy comprises electromagnetic radiation, acoustic energy, or heat.

6. The system of claim 5, wherein the directed energy comprises a series of one or more pulses for controlling a rate of conversion of the biologically active agent from the inactive state to the active state.

7. The system of claim 6, wherein the operations further comprise controlling the rate of conversion based on the determined concentration or abundance of the one or more target analytes.

8. The system of claim 1, wherein the operations further comprise assessing an effect of the biologically active agent on the biological state of the living body based on the determined concentration or abundance of the one or more target analytes.

9. The system of claim 1, wherein the directed energy is applied in response to the determined concentration or abundance of the one or more target analytes.

10. The system of claim 1, wherein the operations further comprise differentiating the analyte response signal from a background signal based, at least in part, on the modulation by the modulation source.

11. The system of claim 1, further comprising an interrogating signal source, wherein the analyte response signal is transmitted in response to an interrogating signal transmitted into the tissue by the interrogating signal source.

12. The system of claim 11, wherein the analyte response signal is modulated by modulating the interrogating signal.

13. The system of claim 1, wherein the tissue comprises subsurface vasculature, and wherein the biologically active agent and functionalized particles are in blood circulating in the subsurface vasculature.

14. The system of claim 1, wherein the wearable device further comprises the directed energy source.

15. A method comprising:

introducing a biologically active agent in an inactive state into a living body, wherein the biologically active agent can be converted to an active state that affects a biological state of the living body;

introducing functionalized particles into the living body, wherein the functionalized particles can interact with one or more target analytes, wherein concentration or abundance of the one or more target analytes in the living body is correlated with the biological state of the living body;

applying directed energy into the living body, wherein the directed energy is sufficient to convert the biologically active agent in the living body from the inactive state to the active state;

detecting, by a wearable device mounted on an external surface of the living body, a signal transmitted from the living body, wherein the signal includes an analyte response signal that is related to interaction of the one or more target analytes with the functionalized particles and an unbound functionalized particle signal that is related to functionalized particles that are not interacting with the one or more target analytes;

applying a modulation into the living body, wherein the modulation can alter the analyte response signal and the unbound functionalized particle signal, such that the analyte response signal is affected differently than the unbound functionalized signal, and

differentiating the analyte response signal from the unbound functionalized particle signal and determining concentration or abundance of the one or more target analytes based on the analyte response signal.

16. The method of claim 15, wherein the directed energy is applied by the wearable device.

17. The method of claim 15, wherein the concentration or abundance of the one or more target analytes is determined after applying the directed energy, further comprising:

assessing an effect of the biologically active agent on the biological state of the living body based on the determined concentration or abundance of the one or more target analytes.

18. The method of claim 15, wherein the concentration or abundance of the target analyte is determined before applying the directed energy, and wherein the directed energy is applied in response to the determined concentration or abundance of the one or more target analytes.

19. The method of claim 15, wherein the biologically active agent comprises a pro-drug when in the inactive state and comprises a drug when in the active state.

20. The method of claim 15, wherein the biologically active agent is associated with a carrier when in the inactive state and is released from the carrier when in the active state.

21. The method of claim 15, wherein the directed energy comprises electromagnetic radiation, acoustic energy, or heat.

22. The method according to claim 15, further comprising differentiating the analyte response signal from a background signal.