Method and system for pre-programmed self-power microfluidic circuits

• US 9,822,890 B2
• Filed: 08/30/2012
• Issued: 11/21/2017
• Est. Priority Date: 08/30/2011
• Status: Active Grant

• Current Assignee: McGill University
• Original Assignee: McGill University
• Inventors: Juncker, David, Safavieh, Hosseinali
• Primary Examiner(s): Le, Minh
• Application Number:
  US14/241,930
• Publication Number:
  US 20140332098A1
• Time in Patent Office: 1,909 Days
• Field of Search: 137825, 137806
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/048 enabling gas exchange, e.g. vents
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: F16K 15/18 with actuating mechanism; Combined check valves and actuated valves
• CPC Class Codes: Y10T 137/7837 Direct response valves [i.e., check valve type]

Abstract

A major challenge for the general use of “lab-on-a-chip” (LOAC) systems and point-of-care (POC) devices has been the generally complex and need for sophisticated peripheral equipment, such that it is more difficult than anticipated to implement low cost, robust and portable LOAC/POC solutions. It would be beneficial for chemical, medical, healthcare, and environmental applications to provide designs for inexpensive LOAC/POC solutions compatible with miniaturization and mass production, and are potentially portable, using compact possibly hand-held instruments, using reusable or disposable detectors. Embodiments of the invention address improved circuit elements for self-powered self-regulating microfluidic circuits including programmable retention valves, programmable trigger valves, enhanced capillary pumps, and flow resonators. Additionally embodiments of the invention allow for the flow direction within a microfluidic circuit to be reversed as well as for retention of reagents prior to sale or deployment of the microfluidic circuit for eased user use.

Claims

1. A microfluidic device for a self-powered microfluidic cicuit including a capillary pump operable to drive a fluid through the self-powered microfluidic circuit, the microfluidic device comprising:

a first microfluidic channel having a first predetermined width, a first predetermined depth, a first end coupled to a first predetermined portion of the microfluidic circuit, and a second distal end coupled to a first end of a third microfluidic channel;

a second microfluidic channel having a second predetermined width over a predetermined section of the second microfluidic channel, a second predetermined depth, a first end coupled to a second end of the third microfluidic channel, and a second distal end coupled to a second predetermined portion of the microfluidic circuit;

the third microfluidic channel disposed between the first and second microfluidic channels, the third microfluidic channel having a third predetermined width and a third predetermined depth;

a cover over the first, second, and third microfluidic channels, the cover comprising a hydrophobic coating on a predetermined region of the cover adjacent the first, second, and third microfluidic channels, the predetermined region comprising at least where the third microfluidic channel intersects the first microfluidic channel;

wherein at least one of the second predetermined width and the second predetermined depth of the second microfluidic channel is selected to establish a predetermined retention pressure for the microfludic device composed of the combination of the first, second, and third microfluidic channels; and

wherein the fluid fed into the first microfluidic channel from the self-powered microfluidic circuit flows by capillary action into the second and third microfluidic channels and is retained therein provided a pressure within the first microfluidic channel, generated by the capillary pump the self-powered microfluidic circuit, is less than the predetermined retention pressure, and wherein when the pressure within the first microfluidic channel exceeds the predetermined retention pressure, the fluid is burst released out of the first end of the second microfluidic channel, through the third microfluidic channel, and into the first microfluidic channel for draining back into the self-powered microfluidic circuit, thereby draining the fluid from the first, second, and third microfluidic channels simultaneously without the fluid being trapped within the third microfluidic channel.

2. The device of claim 1, wherein

the second microfluidic channel has an axis orientated at a first predetermined angle relative to a sidewall of the first microfluidic channel;

and

the third predetermined depth of the third microfluidic channel being less than the second predetermined depth of the second microfluidic channel, the third microfluidic channel having an axis orientated at a second predetermined angle relative to the first predetermined angle of the first microfluidic channel.

3. The device of claim 1, further comprising a cover over the first, second, and third microfluidic channels, the cover comprising a recess situated above the first microfluidic channel such that a sidewall of the recess acts to extend a sidewall of the first microfluidic channel such that the third microfluidic circuit opens essentially into a planar surface.

4. The device of claim 1, wherein the aspect ratio of each of the first, second, and third microfluidic channels is less than 2:

1.

5. The device of claim 1, wherein the aspect ratio of each of the first, second, and third microfluidic channels is less than 3:

1.

6. The device of claim 1, wherein the aspect ratio of each of the first, second, and third microfluidic channels is less than 5:

1.

7. The device of claim 1, wherein the microfluidic circuit includes a microfluidic element in fluid communication with at least one of the first, second and third microfluidic channels, the microfluidic element further comprising:

a first predetermined region including a recess comprising a plurality of posts disposed in a plurality of rows of first predetermined spacing between adjacent rows, second predetermined spacing between adjacent posts within a row, and having a first predetermined angle with respect to an axis of an inlet to the microfluidic element.

8. The device of claim 7, wherein the first and second predetermined spacing and the dimensions of the posts are such that fluid flow from the inlet to an outlet of the microfluidic element occurs primarily along the gaps between adjacent rows of posts rather than between the gaps of adjacent posts within each row.

9. The device of claim 7, wherein the plurality of posts are disposed so that between the plurality of posts and the sidewalls of the recess are open effective channels around the periphery of the recess, each channel having a predetermined width determined in dependence upon establishing predetermined pressure differentials between fluid within each channel and fluid within the region comprising the plurality of posts.

10. The device of claim 9, wherein a first pressure differential between fluid within adjacent rows is higher than a second pressure differential between fluid within a first channel combination leading from the inlet to the outlet and the second pressure differential is higher than a third pressure differential between fluid within a second channel combination leading from the inlet to the outlet, the second channel combination not including channels within the first channel combination.

11. The device of claim 1, further comprising a trigger valve, the trigger valve fluidly coupling the first microfluidic channel and a main fluid channel of the self-powered microfluidic circuit, the trigger valve retaining the fluid within the first microfluidic channel until a second fluid fills the main fluid channel.

12. The device of claim 1, wherein the second microfluidic channel includes a tapering portion having a tapering width along a length of the tapering portion at the first end of the second microfluidic channel, the tapering portion extending between the section of the second microfluidic channel and the third microfluidic channel to provide a fluidicly smooth transition therebetween.

13. The device of claim 1, wherein a transition between the third width of the third microfluidic channel and the first width of the first microfluidic channel is fluidicly abrupt.

14. The device of claim 1, wherein the second width of the second microfluidic channel is greater than the first width of the first microfluidic channel.

15. The device of claim 1, wherein at least one of the third width and the third depth of the third microfluidic channel is greater than the first width and the first depth, respectively, of the first microfluidic channel.

16. A microfluidic device for a self-powered microfluidic circuit including a capillary pump operable to generate a pump capillary pressure to drive a fluid through at least a main fluid channel of the self-powered microfluidic circuit, the microfluidic device comprising:

first, second and third microfluidic channels in serial fluid flow communication and integrally formed with one another, the third microfluidic channel being disposed between the first and second microfluidic channels;

the first microfluidic channel having an first end adapted to communicate with the main fluid channel of the microfluidic circuit such as to receive the fluid therefrom and/or transmit the fluid thereto, a second opposed end of the first microfluidic channel end being connected in fluid communication with an inner end of the third microfluidic channel, the first microfluidic channel having a first width and a first depth;

the second microfluidic channel having a first end connected in fluid communication with an outer end of the third microfluidic channel and a second end adapted to communicate with a vent of the self-powered microfluidic circuit, the second microfluidic channel having a second width over a section of the second microfluidic channel between the first and second ends thereof, the second width of the section of the second microfluidic channel at least partially defining a predetermined retention pressure for the self-powered microfluidic device;

the third microfluidic channel interconnecting the first and second microfluidic channels and having a third width, a third length and a third depth, the third width being greater than the first width of the first microfluidic channel and less than the second width of the portion of the second microfluidic channel;

a cover over the first, second, and third microfluidic channels, the cover comprising a hydrophobic coating on a predetermined region of the cover adjacent the first, second, and third microfluidic channels, the predetermined region comprising at least where the third microfluidic channel intersects the first microfluidic channel; and

wherein the fluid fed into the first microfluidic channel flows by capillary action into the second and third microfluidic channels and is retained therein until a pressure within the first microfluidic channel generated by the capillary pump via the main fluid channel exceeds the predetermined retention pressure, and when the pressure within the first microfluidic channel exceeds the predetermined retention pressure the fluid is burst released out of the first end of the second microfluidic channel, through the third microfluidic channel, and into the first microfluidic channel for draining back into the main fluid channel of the self-powered microfluidic circuit, thereby draining the fluid from the first, second, and third microfluidic channels simultaneously without the fluid being trapped within the third microfluidic channel.

17. The microfluidic device of claim 16, wherein the vent of the self-powered microfluidic circuit is open to atmosphere.

18. The microfluidic device of claim 16, wherein the second microfluidic channel includes a tapering portion having a tapering width along a length of the tapering portion at the first end of the second microfluidic channel, the tapering portion extending between the section of the second microfluidic channel and the third microfluidic channel to provide a fluidicly smooth transition therebetween.

19. The microfluidic device of claim 16, wherein a transition between the third width of the third microfluidic channel and the first width of the first microfluidic channel is fluidicly abrupt.

20. The microfluidic device of claim 16, wherein the second width of the second microfluidic channel is greater than the first width of the first microfluidic channel, and at least one of the third width and the third depth of the third microfluidic channel is greater than the first width and the first depth, respectively, of the first microfluidic channel.

21. The microfluidic device of claim 16, wherein the dimensions of the first microfluidic channel define a first capillary pressure, the dimensions of the second microfluidic channel define a second capillary pressure, and the dimensions of the third microfluidic channel define a third capillary pressure, first capillary pressure being greater than the pump capillary pressure, the second capillary pressure being less than the pump capillary pressure, and the third capillary pressure being greater than the second capillary pressure.