Method, system and apparatus for improved micromanipulation and storage
First Claim
1. Apparatus for micromanipulation of biological material comprising an embryo, said apparatus comprising a vessel having a reservoir with a channel formed in a portion of said reservoir, said channel comprising walls of a thickness in the range of about 0.01 mm to about 0.90 mm and an intermediate restriction dimensioned to resist passage of said biological material but allow passage of liquid treatment solutions comprising cryoprotectants,wherein the apparatus comprises a two-part construction such that two portions of the apparatus are adapted to be heat-sealed with a secondary material intermediate the two portions, the secondary material is a laminate adapted to prevent ingress of LN2 to the apparatus and wherein the secondary material allows for peeling separation of the two part construction.
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Accused Products
Abstract
The present application relates to the manipulation and handling of biological materials and, in one form, provides an apparatus for micromanipulation of biological material, including a channel for accommodating biological material and allowing for passage of liquid treatment solutions. The apparatus may include a two part construction wherein two portions of the apparatus are adapted to be heat sealed with a secondary material intermediate the two portions prior to a vitrification process step. A system for vitrification of a biological specimen is also provided including a software operable means for controlling the temperature environment, a software operable means for controlling fluid dispense volume and velocity and aspiration volume and velocity for the application of liquid treatment solutions to the biological specimen, and a software operable means for controlling protocol time.
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Citations
14 Claims
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1. Apparatus for micromanipulation of biological material comprising an embryo, said apparatus comprising a vessel having a reservoir with a channel formed in a portion of said reservoir, said channel comprising walls of a thickness in the range of about 0.01 mm to about 0.90 mm and an intermediate restriction dimensioned to resist passage of said biological material but allow passage of liquid treatment solutions comprising cryoprotectants,
wherein the apparatus comprises a two-part construction such that two portions of the apparatus are adapted to be heat-sealed with a secondary material intermediate the two portions, the secondary material is a laminate adapted to prevent ingress of LN2 to the apparatus and wherein the secondary material allows for peeling separation of the two part construction.
Specification