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Method, system and apparatus for improved micromanipulation and storage

  • US 9,826,733 B2
  • Filed: 01/07/2014
  • Issued: 11/28/2017
  • Est. Priority Date: 01/07/2013
  • Status: Active Grant
First Claim
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1. Apparatus for micromanipulation of biological material comprising an embryo, said apparatus comprising a vessel having a reservoir with a channel formed in a portion of said reservoir, said channel comprising walls of a thickness in the range of about 0.01 mm to about 0.90 mm and an intermediate restriction dimensioned to resist passage of said biological material but allow passage of liquid treatment solutions comprising cryoprotectants,wherein the apparatus comprises a two-part construction such that two portions of the apparatus are adapted to be heat-sealed with a secondary material intermediate the two portions, the secondary material is a laminate adapted to prevent ingress of LN2 to the apparatus and wherein the secondary material allows for peeling separation of the two part construction.

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