Fluidic systems and methods for analyses
First Claim
1. A multiplex assay system comprising:
- an article supporting solid-phase assays,said article comprising a rigid planar substrate and comprising two or more different liquid containment regions, each liquid containment region comprising at least one analysis region that can be interrogated optically, each analysis region having one or more binding partners associated with a surface of the substrate, wherein said binding partners bind one or more analytes present in a sample in the liquid containment region; and
an optical device configured to supply collimated light to illuminate an analysis region of the two or more liquid containment regions and detect light from the illuminated analysis region, wherein the supplied collimated light and the detected light from the illuminated analysis region travel along a common path.
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Abstract
Fluidic systems and methods for analyses are provided. In some embodiments, systems and methods for improved measurement of absorbance/transmission through fluidic systems are described. Specifically, in one set of embodiments, optical elements are fabricated on one side of a transparent fluidic device opposite a series of fluidic channels. The optical elements may guide incident light passing through the device such that most of the light is dispersed away from specific areas of the device, such as intervening portions between the fluidic channels. By decreasing the amount of light incident upon these intervening portions, the amount of noise in the detection signal can be decreased when using certain optical detection systems.
256 Citations
31 Claims
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1. A multiplex assay system comprising:
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an article supporting solid-phase assays, said article comprising a rigid planar substrate and comprising two or more different liquid containment regions, each liquid containment region comprising at least one analysis region that can be interrogated optically, each analysis region having one or more binding partners associated with a surface of the substrate, wherein said binding partners bind one or more analytes present in a sample in the liquid containment region; and an optical device configured to supply collimated light to illuminate an analysis region of the two or more liquid containment regions and detect light from the illuminated analysis region, wherein the supplied collimated light and the detected light from the illuminated analysis region travel along a common path. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 26, 28, 30)
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23. A multiplex assay system comprising:
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an article supporting solid-phase assays, said article comprising a rigid planar substrate and comprising two or more different liquid containment regions, each liquid containment region comprising at least one analysis region that can be interrogated optically, each analysis region having one or more binding partners associated with a surface of the substrate, wherein said binding partners bind one or more analytes present in a sample in the liquid containment region; and an optical device configured to supply collimated light to illuminate an analysis region of the two or more liquid containment regions and detect light from the illuminated analysis region, wherein the supplied collimated light and the detected light from the illuminated analysis region travel along a common path, wherein each different liquid containment region is a chamber having a depth measured perpendicular to a surface of the rigid planar substrate and a cross-sectional dimension substantially parallel to the surface of the rigid planar substrate, and wherein the rigid planar substrate is composed of glass, quartz, or silicon. - View Dependent Claims (24, 25, 27, 29, 31)
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Specification