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Modified polynucleotides for the production of secreted proteins

  • US 9,828,416 B2
  • Filed: 06/25/2015
  • Issued: 11/28/2017
  • Est. Priority Date: 04/02/2012
  • Status: Active Grant
First Claim
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1. An isolated mRNA comprising;

  • (a) a first region of linked nucleosides, said first region having at least 80% identity to SEQ ID NO;

    5972 encoding a polypeptide of interest, said polypeptide of interest having the sequence of SEQ ID NO;

    2630;

    (b) a first flanking region located at the 5′

    terminus of said first region comprising;

    (i) a sequence of linked nucleosides having the sequence of the native 5′

    UTR of the nucleic acid that encodes SEQ ID NO;

    2630, or having the sequence of SEQ ID NOs;

    1-4; and

    (ii) at least one 5′

    terminal cap;

    (c) a second flanking region located at the 3′

    terminus of said first region comprising;

    (i′

    ) a sequence of linked nucleosides having the sequence of the native 3′

    UTR of the nucleic acid that encodes SEQ ID NO;

    2630, or having the sequence of SEQ ID NOs;

    5-21; and

    (ii′

    ) a 3′

    tailing sequence of linked nucleosides.

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