Modified polynucleotides for the production of secreted proteins
First Claim
Patent Images
1. An isolated mRNA comprising;
- (a) a first region of linked nucleosides, said first region having at least 80% identity to SEQ ID NO;
5972 encoding a polypeptide of interest, said polypeptide of interest having the sequence of SEQ ID NO;
2630;
(b) a first flanking region located at the 5′
terminus of said first region comprising;
(i) a sequence of linked nucleosides having the sequence of the native 5′
UTR of the nucleic acid that encodes SEQ ID NO;
2630, or having the sequence of SEQ ID NOs;
1-4; and
(ii) at least one 5′
terminal cap;
(c) a second flanking region located at the 3′
terminus of said first region comprising;
(i′
) a sequence of linked nucleosides having the sequence of the native 3′
UTR of the nucleic acid that encodes SEQ ID NO;
2630, or having the sequence of SEQ ID NOs;
5-21; and
(ii′
) a 3′
tailing sequence of linked nucleosides.
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Abstract
The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.
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Citations
24 Claims
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1. An isolated mRNA comprising;
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(a) a first region of linked nucleosides, said first region having at least 80% identity to SEQ ID NO;
5972 encoding a polypeptide of interest, said polypeptide of interest having the sequence of SEQ ID NO;
2630;(b) a first flanking region located at the 5′
terminus of said first region comprising;(i) a sequence of linked nucleosides having the sequence of the native 5′
UTR of the nucleic acid that encodes SEQ ID NO;
2630, or having the sequence of SEQ ID NOs;
1-4; and(ii) at least one 5′
terminal cap;(c) a second flanking region located at the 3′
terminus of said first region comprising;(i′
) a sequence of linked nucleosides having the sequence of the native 3′
UTR of the nucleic acid that encodes SEQ ID NO;
2630, or having the sequence of SEQ ID NOs;
5-21; and(ii′
) a 3′
tailing sequence of linked nucleosides. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification