Long-acting polypeptides and methods of producing and administering same
First Claim
1. A method of treating an erythropoietin (EPO)-associated condition, wherein said EPO-associated condition is anemia, said method comprising administering to a subject a therapeutically effective amount of a polypeptide consisting of a chorionic gonadotropin carboxy terminal peptide (CTP) modified erythropoietin (EPO), said CTP-modified EPO polypeptide consisting of one chorionic gonadotropin carboxy terminal peptide (CTP) attached to the amino terminus of said EPO, and two chorionic gonadotropin CTPs attached to the carboxy terminus of said EPO, or said CTP-modified EPO polypeptide consisting of one chorionic gonadotropin carboxy terminal peptide (CTP) attached to the amino terminus of said EPO, and two chorionic gonadotropin CTPs attached to the carboxy terminus of said EPO, and a signal peptide attached to the amino terminal CTP.
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Accused Products
Abstract
A polypeptide and polynucleotides comprising at least two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a non-human peptide-of-interest are disclosed. Pharmaceutical compositions comprising the non-human polypeptides and polynucleotides of the invention and methods of using both human and non-human polypeptides and polynucleotides are also disclosed.
150 Citations
15 Claims
- 1. A method of treating an erythropoietin (EPO)-associated condition, wherein said EPO-associated condition is anemia, said method comprising administering to a subject a therapeutically effective amount of a polypeptide consisting of a chorionic gonadotropin carboxy terminal peptide (CTP) modified erythropoietin (EPO), said CTP-modified EPO polypeptide consisting of one chorionic gonadotropin carboxy terminal peptide (CTP) attached to the amino terminus of said EPO, and two chorionic gonadotropin CTPs attached to the carboxy terminus of said EPO, or said CTP-modified EPO polypeptide consisting of one chorionic gonadotropin carboxy terminal peptide (CTP) attached to the amino terminus of said EPO, and two chorionic gonadotropin CTPs attached to the carboxy terminus of said EPO, and a signal peptide attached to the amino terminal CTP.
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11. A method of stimulating erythropoiesis, said method comprising administering to a subject a therapeutically effective amount of a polypeptide consisting of a chorionic gonadotropin carboxy terminal peptide (CTP) modified erythropoietin (EPO), said CTP-modified EPO consisting of one chorionic gonadotropin carboxy terminal peptide (CTP) attached to the amino terminus of said EPO, and two chorionic gonadotropin CTPs attached to the carboxy terminus of said EPO, and wherein the amino acid sequence of said CTP-modified EPO consists of the sequence set forth in SEQ ID NO:
- 51, or wherein said CTP-modified EPO polypeptide consist of a chorionic gonadotropin carboxy terminal peptide (CTP) modified erythropoietin (EPO), said CTP-modified EPO polypeptide consisting of one chorionic gonadotropin carboxy terminal peptide (CTP) attached to the amino terminus of said EPO, and two chorionic gonadotropin CTPs attached to the carboxy terminus of said EPO, and a signal peptide attached to the amino terminal CTP, and wherein the amino acid sequence of said CTP-modified EPO consists of the sequence set forth in SEQ ID NO;
3. - View Dependent Claims (12, 13, 14, 15)
- 51, or wherein said CTP-modified EPO polypeptide consist of a chorionic gonadotropin carboxy terminal peptide (CTP) modified erythropoietin (EPO), said CTP-modified EPO polypeptide consisting of one chorionic gonadotropin carboxy terminal peptide (CTP) attached to the amino terminus of said EPO, and two chorionic gonadotropin CTPs attached to the carboxy terminus of said EPO, and a signal peptide attached to the amino terminal CTP, and wherein the amino acid sequence of said CTP-modified EPO consists of the sequence set forth in SEQ ID NO;
Specification