Cassette for sample preparation

• US 9,828,598 B2
• Filed: 03/10/2017
• Issued: 11/28/2017
• Est. Priority Date: 10/19/2005
• Status: Active Grant

• Current Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Original Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Inventors: Ching, Jesus, Hu, David Hsiang, Yu, Steve Jia Chang, Lee, Phillip You Fai
• Primary Examiner(s): WHITE, DENNIS MICHAEL
• Application Number:
  US15/456,029
• Publication Number:
  US 20170175106A1
• Time in Patent Office: 263 Days
• CPC Class Codes: B01F 2035/99 Heating
• CPC Class Codes: B01F 2101/23 Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
• CPC Class Codes: B01F 31/65 the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
• CPC Class Codes: B01F 35/712 for feeding fluids
• CPC Class Codes: B01F 35/715 Feeding the components in several steps, e.g. successive steps
• CPC Class Codes: B01F 35/7174 using pistons, plungers or syringes
• CPC Class Codes: B01F 35/90 Heating or cooling systems
• CPC Class Codes: B01L 2200/026 Fluid interfacing between devices or objects, e.g. connectors, inlet details
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 2400/0633 : with moving parts
• CPC Class Codes: B01L 2400/0644 : rotary valves
• CPC Class Codes: B01L 2400/0683 : mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/502 : with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 7/00 : Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: B03C 1/01 : by addition of magnetic adjuvants
• CPC Class Codes: B03C 1/286 : disposed at the inner circumference of a recipient, e.g. magnetic drain bolt
• CPC Class Codes: B03C 2201/18 : Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/20 : Magnetic separation whereby the particles to be separated are in solid form
• CPC Class Codes: C12N 15/1013 : by using magnetic beads
• CPC Class Codes: C12Q 1/6806 : Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 1/38 : Diluting, dispersing or mixing samples
• CPC Class Codes: Y10T 137/0402 : Cleaning, repairing, or assembling
• CPC Class Codes: Y10T 137/0407 : Repairing or assembling hydrant [e.g., fireplug, etc.]
• CPC Class Codes: Y10T 137/0413 : Gas or water meter repairing or assembling
• CPC Class Codes: Y10T 137/0419 : Fluid cleaning or flushing
• CPC Class Codes: Y10T 137/0424 : Liquid cleaning or flushing
• CPC Class Codes: Y10T 436/10 : Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis
• CPC Class Codes: Y10T 436/115831 : Condition or time responsive
• CPC Class Codes: Y10T 436/118339 : with formation of a segmented stream
• CPC Class Codes: Y10T 436/25 : including sample preparation
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 : including use of a solid sorbent, semipermeable membrane, or liquid extraction
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

Apparatuses for preparing a sample are disclosed herein. The apparatuses include a chamber, a first valve at least partially disposed in the first chamber, a second valve at least partially disposed in the first chamber, and a pump comprising an actuator and nozzle.

Claims

1. An apparatus comprising:

(a) a reaction chamber;

(b) a removable lid configured to provide access to an opening in a first surface of the reaction chamber;

(c) a plurality of holding chambers disposed along a second surface of the reaction chamber, wherein each holding chamber of the plurality of holding chambers comprises a valve assembly configured to selectively place the plurality of holding chambers in fluid communication with the reaction chamber via a plurality of openings in the second surface of the reaction chamber;

(d) a particle chamber comprising a first opening and a second opening, wherein the particle chamber and the reaction chamber are in fluid communication with the reaction chamber via the first opening;

(e) a waste chamber comprising an opening, wherein the waste chamber is movable from a first position in which the opening of the waste chamber is aligned with the second opening of the particle chamber and a second position in which the opening of the waste chamber is not aligned with the second opening of the particle chamber; and

(f) an elution chamber comprising an opening, wherein the elution chamber is movable from a first position in which the opening of the elution chamber is aligned with the second opening of the particle chamber and a second position in which the opening of the elution chamber is not aligned with the second opening of the particle chamber.

2. The apparatus of claim 1, further comprising a plunger movable within the reaction chamber.

3. The apparatus of claim 1, wherein the particle chamber contains plastic particles.

4. The apparatus of claim 1, wherein the particle chamber contains cellulose particles.

5. The apparatus of claim 1, wherein the particle chamber contains magnetic particles.

6. The apparatus of claim 1, wherein the elution chamber comprises a second opening and a lid configured to provide access to the second opening of the elution chamber.

7. The apparatus of claim 1, further comprising an overflow waste chamber in fluid communication with the waste chamber.

8. A method for isolating nucleic acid from a sample of cells comprising:

(a) obtaining an apparatus according to claim 1;

(b) placing a sample of cells in the reaction chamber of the apparatus;

(c) transferring a lysis solution from a first holding chamber to the reaction chamber;

(d) transferring a binding solution from a second holding chamber to the reaction chamber;

(e) transferring a bulk material solution comprising the combination of the sample of cells, the lysis solution, and the binding solution from the reaction chamber to the particle chamber and then the waste chamber, wherein nucleic acids present in the bulk material solution bind to particles in the particle chamber and are retained in the particle chamber as the remaining bulk material solution flows into the waste chamber;

(f) transferring a wash solution from a third holding chamber through the reaction chamber, particle chamber, and waste chamber; and

(g) moving the elution chamber into fluid communication with the particle chamber and transferring an elution solution from a fourth holding chamber through the reaction chamber, particle chamber, and the elution chamber, wherein the elution solution releases the nucleic acids from the particles and the nucleic acids flow into the elution chamber with the elution solution.

9. The method of claim 8, further comprising transferring a second wash solution from a fifth holding chamber through the reaction chamber, particle chamber, and waste chamber prior to transferring the elution solution from the fourth holding chamber.

10. The method of claim 8 further comprising removing a lid to access an opening in the elution chamber and removing the nucleic acids from the elution chamber.