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Phage-based bacterial detection assay

  • US 9,828,625 B2
  • Filed: 06/19/2014
  • Issued: 11/28/2017
  • Est. Priority Date: 06/19/2013
  • Status: Active Grant
First Claim
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1. A composition comprising:

  • at least one recombinant phage capable of infecting at least one Listeria or Salmonella microbe obtained from an environmental sample, wherein the at least one recombinant phage comprises a heterologous nucleic acid sequence encoding a marker, and wherein the at least one recombinant phage is selected from the group consisting of LP48;

    ;

    ffluc, LP99;

    ;

    ffluc, LP101;

    ;

    ffluc, LP124;

    ;

    ffluc, LP125;

    ;

    ffluc, LP143;

    ;

    ffluc, A511;

    ;

    ffluc, P100;

    ;

    ffluc, LP40;

    ;

    nluc, LP124;

    ;

    nluc, LP125;

    ;

    nluc, A511;

    ;

    nluc, and P100;

    ;

    nluc; and

    at least one aqueous solution suitable for propagation of the at least one recombinant phage in the at least one Listeria or Salmonella microbe, said solution comprising;

    a) at least one nutrient;

    b) at least one selective agent suitable to inhibit growth of at least one non-target microbe in said environmental sample;

    c) at least one vitamin;

    d) at least one divalent metal;

    e) at least one buffering agent capable of maintaining the composition at pH 7.0-7.5; and

    f) at least one agent suitable to neutralize a sanitizer present in said environmental sample, wherein said agent suitable to neutralize a sanitizer is selected from the group consisting of non-ionic detergents, oxygen scavengers and emulsifiers.

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