Phage-based bacterial detection assay
First Claim
Patent Images
1. A composition comprising:
- at least one recombinant phage capable of infecting at least one Listeria or Salmonella microbe obtained from an environmental sample, wherein the at least one recombinant phage comprises a heterologous nucleic acid sequence encoding a marker, and wherein the at least one recombinant phage is selected from the group consisting of LP48;
;
ffluc, LP99;
;
ffluc, LP101;
;
ffluc, LP124;
;
ffluc, LP125;
;
ffluc, LP143;
;
ffluc, A511;
;
ffluc, P100;
;
ffluc, LP40;
;
nluc, LP124;
;
nluc, LP125;
;
nluc, A511;
;
nluc, and P100;
;
nluc; and
at least one aqueous solution suitable for propagation of the at least one recombinant phage in the at least one Listeria or Salmonella microbe, said solution comprising;
a) at least one nutrient;
b) at least one selective agent suitable to inhibit growth of at least one non-target microbe in said environmental sample;
c) at least one vitamin;
d) at least one divalent metal;
e) at least one buffering agent capable of maintaining the composition at pH 7.0-7.5; and
f) at least one agent suitable to neutralize a sanitizer present in said environmental sample, wherein said agent suitable to neutralize a sanitizer is selected from the group consisting of non-ionic detergents, oxygen scavengers and emulsifiers.
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Abstract
Methods of detecting target bacteria are provided. In some embodiments the methods comprise exposing the sample to a phage capable of infecting a set of target bacteria and comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments the target bacteria comprise Listeria. In some embodiments the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
43 Citations
22 Claims
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1. A composition comprising:
-
at least one recombinant phage capable of infecting at least one Listeria or Salmonella microbe obtained from an environmental sample, wherein the at least one recombinant phage comprises a heterologous nucleic acid sequence encoding a marker, and wherein the at least one recombinant phage is selected from the group consisting of LP48;
;
ffluc, LP99;
;
ffluc, LP101;
;
ffluc, LP124;
;
ffluc, LP125;
;
ffluc, LP143;
;
ffluc, A511;
;
ffluc, P100;
;
ffluc, LP40;
;
nluc, LP124;
;
nluc, LP125;
;
nluc, A511;
;
nluc, and P100;
;
nluc; andat least one aqueous solution suitable for propagation of the at least one recombinant phage in the at least one Listeria or Salmonella microbe, said solution comprising; a) at least one nutrient; b) at least one selective agent suitable to inhibit growth of at least one non-target microbe in said environmental sample; c) at least one vitamin; d) at least one divalent metal; e) at least one buffering agent capable of maintaining the composition at pH 7.0-7.5; and f) at least one agent suitable to neutralize a sanitizer present in said environmental sample, wherein said agent suitable to neutralize a sanitizer is selected from the group consisting of non-ionic detergents, oxygen scavengers and emulsifiers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification