Detection device and methods of use

• US 9,828,632 B2
• Filed: 11/12/2014
• Issued: 11/28/2017
• Est. Priority Date: 02/05/2007
• Status: Active Grant

• Current Assignee: QIAGEN Sciences, LLC (Qiagen NV)
• Original Assignee: Intelligent Bio-Systems Incorporated. (Qiagen NV)
• Inventors: Gordon, Steven, Auger, Steven, Schinazi, Alan
• Primary Examiner(s): Kim, Young J
• Application Number:
  US14/539,244
• Publication Number:
  US 20150159211A1
• Time in Patent Office: 1,112 Days
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/6471 Special filters, filter wheel
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence

Abstract

An imaging system for exciting and measuring fluorescence on or in samples comprising fluorescent materials (e.g. fluorescent labels, dyes or pigments). In one embodiment, a device is used to detect fluorescent labels on nucleic acid. In a preferred embodiment, the device is configured such that fluorescent labels in a plurality of different DNA templates are simultaneously detected.

Claims

1. A method of imaging fluorescent signals, comprising:

a) providing i) a first array in a first flow cell, said first array comprising a plurality of different nucleic acid templates, ii) a second array in a second flow cell, said second array comprising a plurality of different nucleic acid templates, said first and second flow cells positioned on a moving support, iii) nucleic acid sequencing reagents capable of producing fluorescent signals from fluorescent labels covalently attached to a nucleotide that can be incorporated in said nucleic acid, and iv) a single camera, wherein said first and second flow cells comprise a fluid channel and said nucleic acid templates are arrayed on said fluid channel, said fluid channel in fluidic communication with a side entrance port;

b) introducing nucleic acid sequencing reagents into said first flow cell via said side entrance port under conditions so as to generate first fluorescent signals in said first array;

c) introducing nucleic acid sequencing reagents into said second flow cell via said side entrance port under conditions such that, while said second array in said second flow cell is undergoing one or more reaction steps so as to produce fluorescent signals, said first array is being imaged with said single camera;

d) moving said first flow cell on said moving support; and

e) moving said second flow cell on said moving support and imaging said second array in said second flow cell with said single camera.

2. The method of claim 1, wherein said moving support is a rotary stage.

3. The method of claim 1, further comprising f) draining said fluid channel of said first flow cell at least in part by gravity.

4. The method of claim 1, wherein reagent and buffer reservoirs are in communication with said first and second flow cells via said side entrance port.