Electrophoresis running tank assembly

• US 9,835,587 B2
• Filed: 04/01/2014
• Issued: 12/05/2017
• Est. Priority Date: 04/01/2014
• Status: Active Grant

• Current Assignee: CC IMEX
• Original Assignee: C.C. Imex
• Inventors: Walker, Winston Glenn, Chan, Richard, Wong, Rita M., Chan, Ramey K., Cheng, Po Ling
• Primary Examiner(s): Merkling, Sally A
• Application Number:
  US14/242,580
• Publication Number:
  US 20150276673A1
• Time in Patent Office: 1,344 Days
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

An electrophoresis running tank assembly uses two opposed rows of LEDs to illuminate DNA-containing gel on a transparent tray positioned between the rows. A respective cylindrical lens is positioned horizontally between each row and a respective edge of the tray. The optical axis of the illumination light is midway between a bottom surface of the gel tray and a top surface of the gel.

Claims

1. Assembly for electrophoresis, comprising:

at least one tank formed with a gel tray platform including a top surface configured for holding at least one gel tray containing gel;

at least an anode reservoir on a first side of the gel tray platform and at least a cathode reservoir on a second side of the gel tray platform, both reservoirs configured for holding buffer during electrophoresis, the anode reservoir being larger than the cathode reservoir, both reservoirs having a respective closed bottom, a respective top opposed to the respective closed bottom, and a respective buffer chamber therebetween, the anode reservoir and the cathode reservoir extending laterally inward under the gel tray;

at least an anode in the anode reservoir and at least a cathode in the cathode reservoir, the anode and cathode being disposed in the respective buffer chamber and spaced above the respective closed bottom, a first distance being established between the anode and a side of the gel tray facing the anode and at least a second distance being established between the cathode and a side of the gel tray facing the cathode, the first distance being greater than the second distance;

at least a first source of illumination facing the platform;

at least a second source of illumination facing the platform and facing the first source of illumination with the platform between the first and second sources of illumination, the first and second sources of illumination positioned to emit light along respective first and second central light axes that are coplanar with each other and that are parallel to and spaced from the top surface of the gel tray platform, the top of the gel tray platform being a horizontal top on top of which is located a shelf platform with a top surface that is at least partially speculative and opaque and configured for receiving the gel tray thereon.

2. The assembly of claim 1, comprising:

at least a first lens positioned between the first source of illumination and the platform to focus light from the first source of illumination in a pattern defining a first central light axis; and

at least a second lens positioned between the second source of illumination and the platform to focus light from the second source of illumination in a pattern defining a second central light axis.

3. The assembly of claim 1, wherein the anode and cathode are made of carbon.

4. The assembly of claim 1, wherein the anode and cathode are elongated and define respective longitudinal axes parallel to the closed bottoms of the respective buffer chambers.

5. The assembly of claim 2, wherein the first lens is an elongated horizontally-oriented cylindrical lens.

6. The assembly of claim 2, wherein the first source of illumination is recessed radially outward of a surface with which the first lens is mounted.

7. The assembly of claim 1, wherein the first source of illumination has a flat distal end through which light emerges.

8. The assembly of claim 1, wherein the first and second sources of illumination face each other.

9. The assembly of claim 1, wherein at least the cathode is laterally spaced from the gel tray by a distance less than three millimeters.

10. Assembly for electrophoresis, comprising:

at least one tank holding a gel tray platform assembly including a top surface configured for holding at least one gel tray containing gel with DNA therein;

at least a first light emitting diode (LED) juxtaposed with a first wall of the tank, the first wall facing the platform;

at least a second LED juxtaposed with a second wall of the tank, the second wall facing the platform;

at least a first optical element positioned between the first LED and the platform to pass light from the first LED to the gel tray platform;

a gel tray with a clear bottom wall defining a bottom surface, the bottom wall supporting a gel defining a top gel surface, the gel tray being positioned on the top surface of the gel tray platform assembly, respective first and second light axes from the respective first and second LEDs being coplanar with a plane midway between the top surface of the gel and the bottom surface of the gel tray, the top surface of the gel tray platform assembly being at least partially speculative and opaque.

11. The assembly for electrophoresis of claim 10, comprising a filter removably engageable with the tank to filter light from the LED from an observer of the gel while allowing emitted wavelengths from the stain to pass through to the observer, the filter having a top of opaque filter material with a central transparent or translucent colored window, the top being parallel to the top surface of the gel tray platform assembly.

12. The assembly for electrophoresis of claim 10, comprising:

at least a second lens positioned between the second LED and the gel tray platform assembly to focus light from the second LED along the light axis.

13. The assembly for electrophoresis of claim 10, comprising:

at least an anode reservoir on a first side of the gel tray platform assembly and at least a cathode reservoir on a second side of the gel tray platform assembly, both reservoirs configured for holding buffer during electrophoresis;

at least an anode in the anode reservoir and at least a cathode in the cathode reservoir, a first distance being established between the anode and a side of the gel tray facing the anode when the gel tray is positioned on the gel tray platform assembly and at least a second distance being established between the cathode and a side of the gel tray facing the cathode when the gel tray is positioned on the gel tray platform assembly.

14. The assembly for electrophoresis of claim 13, wherein the first distance is greater than the second distance.

15. The assembly for electrophoresis of claim 13, wherein the anode reservoir is larger than the cathode reservoir.

16. The assembly for electrophoresis of claim 10, wherein the optical element is a first lens and first LED is one of a first group of LEDs oriented in a horizontal row and recessed below a surface onto which the first lens is mounted and the second LED is one of a second group of LEDs oriented in a horizontal row and recessed below a surface onto which the second lens is mounted.

17. The assembly for electrophoresis of claim 16, wherein the first and second groups of LEDs face each other.

18. Assembly for electrophoresis for allowing an operator to observe the progress of DNA bands as they migrate and separate comprising:

at least a first reservoir for buffer with a cathode element therein;

at least a second reservoir for buffer with an anode element therein;

the cathode element and anode element being configured for connection to at least one source of voltage;

at least one gel tray platform between the elements and configured for supporting at least one gel tray containing a gel having DNA therein;

at least a first source of illumination juxtaposed with a first side of the gel tray platform and positioned to emit light along a light axis, the gel tray platform having a horizontal top on top of which is located a shelf platform with a top surface that is at least partially speculative and opaque, the gel tray resting on the shelf platform and

at least a second source of illumination juxtaposed with a second side of the gel tray platform, the second side of the gel tray platform not being the first side of the gel tray platform.

19. The assembly of claim 18, wherein the cathode element is positioned near a proximate edge of the gel and the anode element is positioned near a distal edge of the gel that is opposite to the proximal edge.

20. The assembly of claim 18, wherein, the light axis is co-planar with a plane that is midway between a top surface of the gel and a bottom surface of the gel tray.

21. The assembly of claim 18, wherein the light axis is orthogonal to a line intersecting the anode and cathode.

22. The assembly of claim 18, wherein the gel tray is made of clear plastic such that a bottom of the gel tray in cooperation with the top surface of the gel shelf platfo establishes a light pipe to excite the stain associated with the DNA.

23. The assembly of claim 18, wherein the first source of illumination comprises a blue light emitting diode (LED) and the gel comprises a green stain.

24. The assembly of claim 18, comprising a lens positioned between the first source of illumination and the gel platform and located to focus light from the first source of illumination on a first edge of the gel.

25. The assembly of claim 18, comprising an opening in a wall of the assembly between the first source of illumination and the gel platform and a lens extending inwardly past the opening and located to focus light from the first the source of illumination on a first edge of the gel, the lens being coupled to a transparent block disposed in the opening to at least partially seal the first source of illumination from fluid in the first reservoir.

26. The assembly of claim 18, comprising a filter configured to filter light from the at least one source of illumination from an observer of the gel while allowing emitted wavelengths from the stain to pass through to the observer.

27. The assembly of claim 26, wherein the filter is established by a hood with a top of filter material for mounting a camera.

28. The assembly of claim 27, wherein at least one side of the hood is formed with filter material to allow observation simultaneously while running the gel.