Rapid small volume detection of blood ammonia

• US 9,835,613 B2
• Filed: 10/14/2016
• Issued: 12/05/2017
• Est. Priority Date: 02/11/2014
• Status: Active Grant

• Current Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford University)
• Original Assignee: Board of Trustees of the Leland Stanford Junior University (Stanford University)
• Inventors: Veltman, Thomas Richard, Tsai, Chun J., Kanan, Matthew William, Chu, Gilbert
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US15/293,935
• Publication Number:
  US 20170030893A1
• Time in Patent Office: 417 Days
• CPC Class Codes: G01N 27/4045 for gases other than oxygen
• CPC Class Codes: G01N 27/4074 for detection of gases other than oxygen
• CPC Class Codes: G01N 2800/04 Endocrine or metabolic disorders
• CPC Class Codes: G01N 33/0054 Ammonia
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02
• CPC Class Codes: G01N 33/48707 by electrical means G01N33/49, G01N33/493 take precedence
• CPC Class Codes: G01N 33/4925 measuring blood gas content, e.g. O2, CO2 , HCO3
• CPC Class Codes: Y02A 50/20 Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

A method for measuring ammonia in a blood sample may involve positioning the blood sample in proximity with an ammonia gas sensor, generating a current with the ammonia gas sensor in response to ammonia gas released from the blood sample, and measuring the current generated by the ammonia gas sensor, using a current measurement member coupled with the ammonia gas sensor. A device for measuring an ammonia level in a blood sample may include a blood sample containment member, an ammonia gas sensor coupled with the blood sample containment member, and a current measurement member coupled with the ammonia gas sensor. The method and device may be used to measure an ammonia level in a blood sample as small as one drop of blood, or approximately 0.05 mL of blood.

Claims

1. A method for measuring ammonia in a blood sample, the method comprising:

positioning the blood sample in a sealed chamber that surrounds at least part of an anode end of an ammonia fuel cell, so the anode end is exposed to ammonia gas released from the blood sample;

generating a current with the ammonia fuel cell in response to the ammonia gas released from the blood sample; and

measuring the current generated by the ammonia fuel cell, using a current measurement member coupled with the ammonia fuel cell.

2. A method as in claim 1, wherein positioning the blood sample further comprises attaching a blood sample containment member to the ammonia fuel cell to form the sealed chamber.

3. A method as in claim 2, wherein attaching the blood sample containment member to the ammonia fuel cell forms at least one aperture in the blood sample containment member to form the sealed chamber.

4. A method as in claim 1, wherein the blood sample comprises no more than 0.05 mL of blood.

5. A method as in claim 1, further comprising mixing the blood sample with an alkaline substance before generating the current.

6. A method as in claim 5, wherein mixing the blood sample is performed before positioning the blood sample.

7. A method as in claim 5, wherein mixing the blood sample is performed after positioning the blood sample.

8. A method as in claim 5, wherein the alkaline substance is selected from the group consisting of:

potassium carbonate;

an aqueous solution of lithium chloride and lithium hydroxide;

other hydroxides; and

other salts.

9. A method as in claim 5, further comprising stirring or agitating the blood sample and the alkaline substance at least one of during or after the mixing step.

10. A method as in claim 1, wherein positioning the blood sample comprises positioning a substrate holding the blood sample in proximity with the anode end of the ammonia fuel cell.

11. A method as in claim 1, wherein measuring the current comprises measuring with an ammeter.

12. A method as in claim 1, wherein measuring the current comprises measuring with a potentiostat.

13. A method as in claim 1, further comprising displaying the measured current on a display member coupled with the current measurement member.

14. A method as in claim 1, wherein the method is performed using a portable ammonia measurement device comprising a blood sample containment member, the ammonia fuel cell coupled with the blood sample containment member, and the current measurement member coupled with the ammonia fuel cell, and wherein the portable ammonia measurement device is configured to be held in one hand of a user.

15. A device for measuring an ammonia level in a blood sample, the device comprising:

a blood sample containment member comprising a compartment for holding the blood sample;

an ammonia gas sensor coupled with the blood sample containment member, wherein the compartment forms a sealed chamber when the blood sample containment member is coupled with the ammonia gas sensor, and wherein the sealed chamber encloses an anode end of the ammonia gas sensor and the blood sample; and

a current measurement member coupled with the ammonia gas sensor.

16. A device as in claim 15, wherein the ammonia gas sensor comprises an ammonia fuel cell.

17. A device as in claim 15, wherein the blood sample containment member is removably coupled with the ammonia gas sensor.

18. A device as in claim 15, wherein the current measurement member comprises an electric circuit coupled at one end with the anode end of the ammonia gas sensor and at another end with a cathode end of the ammonia gas sensor.

19. A device as in claim 15, wherein the blood sample containment member comprises a substrate for holding the blood sample.

20. A device as in claim 19, further comprising a substance pre-applied onto the substrate before application of the blood sample to the substrate.

21. A device as in claim 20, wherein the substance is selected from the group consisting of:

potassium carbonate;

an aqueous solution of lithium chloride and lithium hydroxide;

other hydroxides; and

other salts.

22. A device as in claim 15, wherein the current measurement member comprises an ammeter.

23. A device as in claim 15, wherein the current measurement member comprises a potentiostat.

24. A device as in claim 15, further comprising a housing at least partially containing the ammonia gas sensor and the current measurement member, wherein the blood sample containment member is removably couplable with the housing.

25. A device as in claim 24, wherein the blood sample containment member comprises a container couplable with the housing to form a sealed chamber in which the blood sample is contained.

26. A device as in claim 25, wherein the blood sample containment member comprises a sealed vial comprising a membrane, and wherein coupling the blood sample containment member with the housing pierces the membrane and forms the sealed chamber.

27. A device as in claim 24, wherein the blood containment member comprises a substrate, and wherein the housing comprises a slot into which the substrate is advanced to expose the blood sample to the ammonia gas sensor.

28. A device as in claim 24, further comprising a display on the housing for displaying the measured blood ammonia level.

29. A device as in claim 28, wherein the display is configured to display at least one of a numerical indication or a linear representation of the measured blood ammonia level.

30. A device as in claim 15, further comprising a two-part housing, wherein a first part of the two-part housing at least partially contains the ammonia gas sensor and the current measurement member, and wherein a second part of the two-part housing at least partially contains the blood sample containment member.

31. A device as in claim 15, wherein the blood sample comprises no more than 0.05 mL of blood.

32. A device as in claim 15, wherein the device is sufficiently small to be held in one hand of a user while in use.