Sensitive and rapid determination of antimicrobial susceptibility
First Claim
1. A system for the identification of individual microorganisms in a sample comprising:
- an enclosed chamber comprising;
a first electrode disposed on a detection surface, wherein the first electrode is a transparent conductive surface;
a second electrode disposed on a second surface;
an input port configured to transport a fluid into the chamber;
an output port configured to transport the fluid out of the chamber;
a capture surface disposed on the first electrode, wherein the capture surface comprises a binding agent configured to bind the individual microorganisms in spatially discrete locations;
an electrical controller operably linked to the first and second electrodes and configured to control potential between the first and second electrodes;
an optical detector configured to detect the individual microorganisms bound to the capture surface; and
a storage controller configured to perform analysis of an image obtained by the optical detector.
5 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to moving microorganisms to a surface, where they are grown in the presence and absence of antimicrobials, and by monitoring the growth of the microorganisms over time in the two conditions, their susceptibility to the antimicrobials can be determined. The microorganisms can be moved to the surface through electrophoresis, centrifugation or filtration. When the movement involves electrophoresis, the presence of oxidizing and reducing reagents lowers the voltage at which electrophoretic force can be generated and allows a broader range of means by which the target can be detected. Monitoring can comprise optical detection, and most conveniently includes the detection of individual microorganisms. The microorganisms can be stained in order to give information about their response to antimicrobials.
-
Citations
9 Claims
-
1. A system for the identification of individual microorganisms in a sample comprising:
-
an enclosed chamber comprising;
a first electrode disposed on a detection surface, wherein the first electrode is a transparent conductive surface;
a second electrode disposed on a second surface;
an input port configured to transport a fluid into the chamber;
an output port configured to transport the fluid out of the chamber;a capture surface disposed on the first electrode, wherein the capture surface comprises a binding agent configured to bind the individual microorganisms in spatially discrete locations; an electrical controller operably linked to the first and second electrodes and configured to control potential between the first and second electrodes; an optical detector configured to detect the individual microorganisms bound to the capture surface; and a storage controller configured to perform analysis of an image obtained by the optical detector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
Specification