×

Nanonozzle device arrays: their preparation and use for macromolecular analysis

  • US 9,845,238 B2
  • Filed: 05/14/2015
  • Issued: 12/19/2017
  • Est. Priority Date: 07/19/2006
  • Status: Active Grant
First Claim
Patent Images

1. A method for characterizing one or more features of a macromolecule, comprising:

  • linearizing the macromolecule residing at least in part within a nanochannel, said macromolecule comprising a label,at least a portion of the nanochannel being capable of physically constraining at least a portion of the macromolecule so as to maintain in linear form that portion of the macromolecule, andwherein a first effective inner diameter of the nanochannel is between about 10 nm and about 500 nm, the nanochannel comprising at least one constriction that is configured to locally reduce the effective inner diameter to a second effective inner diameter of about 0.5 nm to about 100 nm, whereby the second effective inner diameter is less than the first effective inner diameter; and

    transporting at least a portion of the macromolecule within at least the portion of the nanochannel such that at least the portion of the macromolecule passes through the constriction;

    monitoring at least one signal arising in connection with the label of the macromolecule passing through the constriction,wherein the at least one signal characterizes one or more features of the macromolecule.

View all claims
  • 11 Assignments
Timeline View
Assignment View
    ×
    ×