Assay devices with integrated sample dilution and dilution verification and methods of using same
First Claim
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1. A method of performing an assay for an analyte in a sample, said method comprising the steps of:
- introducing the sample into a sample chamber of a cartridge, wherein the sample chamber terminates in a sample extraction unit;
loading said extraction unit with said sample;
washing a portion of said sample from the extraction unit by pumping a volume of diluent from a diluent conduit through the extraction unit to form a diluted sample;
analyzing said diluted sample for an analyte; and
determining a dilution ratio of the diluted sample.
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Abstract
The invention is to devices and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature for forming a diluted sample for analysis. The devices and methods also include a dilution verification feature for verifying the degree of dilution of the diluted sample. The devices preferably are capable of being used in the point-of-care diagnostic field is provided.
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Citations
20 Claims
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1. A method of performing an assay for an analyte in a sample, said method comprising the steps of:
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introducing the sample into a sample chamber of a cartridge, wherein the sample chamber terminates in a sample extraction unit; loading said extraction unit with said sample; washing a portion of said sample from the extraction unit by pumping a volume of diluent from a diluent conduit through the extraction unit to form a diluted sample; analyzing said diluted sample for an analyte; and determining a dilution ratio of the diluted sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification