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Enhanced production of lipids containing polyenoic fatty acids by very high density cultures of eukaryotic microbes in fermentors

  • US 9,848,623 B2
  • Filed: 01/03/2012
  • Issued: 12/26/2017
  • Est. Priority Date: 01/28/2000
  • Status: Expired due to Fees
First Claim
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1. A process for producing microbial lipids comprising conducting a fermentation of microorganisms of the order Thraustochytriales capable of producing at least 15% of lipids as highly unsaturated fatty acids having at least four unsaturated carbon-carbon bonds in a fermentation medium comprising a carbon source and a limiting nutrient source, the process comprising:

  • a) providing the fermentation medium comprising the carbon source and limiting nutrient source;

    b) adding the microorganisms to the fermentation medium;

    c) adding an additional amount of the carbon source and limiting nutrient source to a fermentation medium to increase the biomass density of the fermentation medium to at least 100 g/L on a dry cell weight basis; and

    d) adding an additional amount of the carbon source while limiting the amount of the limiting nutrient source added to the formulation medium to induce lipid production by the microorganisms.

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