Perfusion assessment using transmission laser speckle imaging
First Claim
1. An apparatus for determining a metric of tissue perfusion, comprising:
- a coherent light source configured to illuminate a tissue sample;
a detector, without a lens, system of lenses, mirror or any other optical instruments for creating a focused image, wherein the detector is configured to receive a plurality of light intensity values associated with an unfocused image of the tissue sample; and
an enclosure configured to accommodate the tissue sample, wherein the enclosure is coupled to the coherent light source and the detector,wherein the coherent light source and the detector are configured in a transmission geometry with respect to the tissue sample, andwherein the detector is configured to transfer the plurality of light intensity values to a processor configured to compute a speckle contrast parameter and to determine the metric of tissue perfusion.
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Accused Products
Abstract
Methods and apparatus for measuring perfusion using transmission laser speckle imaging are provided. The apparatus comprises a coherent light source and a detector configured to measure transmitted light associated with an unfocused image at one or more locations. The coherent light source and detector are positioned in a transmission geometry. The apparatus further comprises means for securing the coherent light source and the detector to the tissue sample in a fixed transmission geometry relative to the tissue sample. The apparatus may further comprise at least one processor to receive information from the detector and process detected variations in transmitted light intensity to determine a single metric of perfusion. The method may comprise the steps transilluminating a tissue sample with coherent light, recording spatial and/or temporal variations in the transmitted light signal, determining speckle contrast value(s), and computing a metric of perfusion.
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Citations
21 Claims
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1. An apparatus for determining a metric of tissue perfusion, comprising:
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a coherent light source configured to illuminate a tissue sample; a detector, without a lens, system of lenses, mirror or any other optical instruments for creating a focused image, wherein the detector is configured to receive a plurality of light intensity values associated with an unfocused image of the tissue sample; and an enclosure configured to accommodate the tissue sample, wherein the enclosure is coupled to the coherent light source and the detector, wherein the coherent light source and the detector are configured in a transmission geometry with respect to the tissue sample, and wherein the detector is configured to transfer the plurality of light intensity values to a processor configured to compute a speckle contrast parameter and to determine the metric of tissue perfusion. - View Dependent Claims (2, 3, 4, 5, 6)
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7. An apparatus for determining a metric of tissue perfusion, comprising:
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a coherent light source configured to illuminate a tissue sample; a detector, without a lens, system of lenses, mirror or any other optical instruments for creating a focused image, wherein the detector is configured to receive a plurality of light intensity values associated with an unfocused image of the tissue sample; and means for securing the coherent light source and the detector to the tissue sample in a fixed transmission geometry relative to the tissue sample, such that movement of the tissue sample while measuring tissue perfusion does not alter the transmission geometry, wherein the detector is configured to transfer the plurality of light intensity values to a processor configured to compute a speckle contrast parameter and to determine the metric of tissue perfusion. - View Dependent Claims (8, 9, 10, 11, 12, 13, 20, 21)
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14. A method of determining a metric of perfusion in a tissue sample, comprising:
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providing an apparatus comprising an enclosure configured to accommodate the tissue sample, a coherent light source configured to illuminate the tissue sample, and a detector, without a lens, system of lenses, mirror or any other optical instruments for creating a focused image, wherein the detector is configured to receive a plurality of light intensity values associated with an unfocused image of the tissue sample, and wherein the detector is configured to transfer data to a processor; placing the tissue sample into the enclosure between the coherent light source and the detector; transilluminating the tissue sample with the coherent light source rendering an unfocused image comprising a plurality of light intensity values, which are captured by the detector; transferring the light intensity values from the detector to the processor; computing, by the processor, a speckle contrast parameter based at least in part on the plurality of light intensity values; and determining, by the processor, the metric of perfusion from the speckle contrast parameter. - View Dependent Claims (15, 16, 17, 18, 19)
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Specification