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Discrete imaging of hepatic oxidative and nitrosative stress with two-channel nanoparticles for in vivo drug safety screening

  • US 9,849,198 B2
  • Filed: 07/02/2014
  • Issued: 12/26/2017
  • Est. Priority Date: 07/02/2013
  • Status: Active Grant
First Claim
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1. A bifunctional nanoprobe comprising:

  • (i) a matrix core comprising;

    (a) a fluorescent superconducting polymer, wherein said polymer is a 9,9 diarylpolyfluorene polymer or a poly(p-phenylene vinylene) polymer; and

    (b) a copolymer comprised of polystyrene and polyethylene glycol monomers and having a plurality of galactose moieties conjugated to the polyethylene glycol and wherein the plurality of galactose moieties are disposed at the surface of the matrix core;

    (ii) a chemiluminescent sensor cleavable by hydrogen peroxide and selected from the group consisting of bis-(2,4,5-trichloro-6-(pentyloxycarbonyl)phenyl)oxalate (CPPO), 6-(4-methoxyphenyl)-2-methyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA), and 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one (CLA); and

    (iii) a fluorescent sensor, wherein said fluorescent sensor is 2-[4′

    -(β

    -carboxyethylthio)-7′

    -(1″

    ,3″

    ,3″

    -trimethylindolenine)-3′

    ,5′

    -trimethyleneheptatrien-1-yl]-1,3,3-trimethylindolenium perchlorate (IR775S) or diaminocyanine.

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