Heat flow polymerase chain reaction systems and methods
First Claim
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1. A system, comprising:
- a sample block including a plurality of sample wells;
at least one heater coupled to the sample block and configured to add heat to at least one PCR reaction solution when the at least one PCR reaction solution is disposed within a sample well from the plurality of sample wells and to add heat to at least one reference solution when the at least one reference solution is disposed within a sample well from the plurality of sample wells;
a first temperature sensor configured and disposed to measure a temperature of a PCR reaction solution during each of a plurality of cycles when the PCR reaction solution is disposed within a first sample well from the plurality of sample wells;
a second temperature sensor configured and disposed to measure a temperature of a reference solution during each of the plurality of cycles when the reference solution is disposed within a second sample well from the plurality of sample wells; and
a computer controller in communication with the first temperature sensor and the second temperature sensor, the computer controller programmed to compare a temperature of the PCR reaction solution to a temperature of the thermal reference solution measured during each cycle from the plurality of cycles and to detect a formation of amplified DNA within the PCR reaction solution when a difference between a temperature of the PCR reaction solution and a temperature of the thermal reference solution is greater than a threshold temperature difference.
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Abstract
Methods and systems for polymerase chain reactions (PCR) that are capable of detecting amplified DNA during or after the PCR process. The methods and systems may utilize DSC or DTA analysis techniques.
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Citations
21 Claims
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1. A system, comprising:
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a sample block including a plurality of sample wells; at least one heater coupled to the sample block and configured to add heat to at least one PCR reaction solution when the at least one PCR reaction solution is disposed within a sample well from the plurality of sample wells and to add heat to at least one reference solution when the at least one reference solution is disposed within a sample well from the plurality of sample wells; a first temperature sensor configured and disposed to measure a temperature of a PCR reaction solution during each of a plurality of cycles when the PCR reaction solution is disposed within a first sample well from the plurality of sample wells; a second temperature sensor configured and disposed to measure a temperature of a reference solution during each of the plurality of cycles when the reference solution is disposed within a second sample well from the plurality of sample wells; and a computer controller in communication with the first temperature sensor and the second temperature sensor, the computer controller programmed to compare a temperature of the PCR reaction solution to a temperature of the thermal reference solution measured during each cycle from the plurality of cycles and to detect a formation of amplified DNA within the PCR reaction solution when a difference between a temperature of the PCR reaction solution and a temperature of the thermal reference solution is greater than a threshold temperature difference. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A system, comprising:
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a sample block including a plurality of sample wells a first heating system coupled to the sample block and configured to add heat to a PCR reaction solution when the PCR reaction solution is disposed within a first sample well from the plurality of sample wells; a second heating system coupled to the sample block and configured to add heat to a reference solution when the reference solution is disposed within a second sample well from the plurality of sample wells; a first temperature sensor configured and disposed to measure a temperature of the PCR reaction solution during each of a plurality of cycles when the PCR reaction solution is disposed within the first sample well from the plurality of sample wells; a second temperature sensor configured and disposed to measure a temperature of the reference solution during each of the plurality of cycles when the reference solution is disposed within the second sample well from the plurality of sample wells; and a computer controller in communication with the first heating system and the second heating system, the computer controller programmed to detect a formation of amplified DNA within the PCR reaction solution based on a difference in a power input to the first heating system and a power input to the second heating system. - View Dependent Claims (10, 11, 12, 13)
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14. A system, comprising:
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a sample block including a plurality of sample wells a heater coupled to the sample block and configured to add heat to at least one PCR reaction solution when the at least one PCR reaction solution is disposed within a sample well from the plurality of sample wells and to add heat to at least one reference solution when the at least one reference solution is disposed within a sample well from the plurality of sample wells; a first temperature sensor configured and disposed to measure a temperature of a PCR reaction solution during each of a plurality of cycles when the PCR reaction solution is disposed within a first sample well from the plurality of sample wells; a second temperature sensor configured and disposed to measure a temperature of the reference solution during each of the plurality of cycles when the reference solution is disposed within a second sample well from the plurality of sample wells; and a computer controller in communication with the first temperature sensor and the second temperature sensor, the computer controller programmed to compare a temperature of a PCR reaction solution to a temperature of a thermal reference solution during at least one cycle from the plurality of cycles and to determine an enthalpy change in the PCR reaction solution, the computer controller programmed to relate a difference in temperature between the PCR reaction solution and the thermal reference solution to the enthalpy change in the PCR reaction solution to detect a formation of amplified DNA within the PCR reaction solution. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21)
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Specification