Device and methods for detection of analytes including use of a colorimetric barcode
First Claim
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1. A method for determining an analyte, comprising:
- introducing a sample suspected of containing an analyte into a sample inlet of a sample analysis device, the sample analysis device comprising a plurality of channels constructed and arranged to receive the sample suspected of containing an analyte from the sample inlet, each channel comprising a different chemical reagent, each chemical reagent capable of generating a luminescent or colorimetric signal;
treating the sample with a plurality of different solvents provided from a corresponding plurality of solvent chambers disposed within the sample analysis device, wherein the plurality of solvent chambers are in fluid communication with the sample inlet; and
simultaneously introducing the treated sample into the plurality of channels such that the analyte, if present, interacts with one or more of the chemical reagents to produce a plurality of luminescent or colorimetric signals, thereby determining the analyte, wherein the plurality of channels are in fluid communication with the sample inlet, andwherein the presence and/or identity of the analyte is determined without need for a non-luminescent or non-colorimetric analysis of the sample.
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Abstract
Embodiments described herein related to devices and methods for the collection and/or determination of analytes, such as illicit substances including military explosives, explosives, and precursors thereof. In some cases, the device may be a disposable device that incorporates highly efficient sample collection in combination with microfluidic-based chemical analysis resulting in the rapid detection and identification of unknown materials. In some cases, multiple colorimetric detection chemistries may be employed, and the resulting “barcode” of color changes can be used to positively identify the presence and/or identity of the analyte.
20 Citations
24 Claims
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1. A method for determining an analyte, comprising:
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introducing a sample suspected of containing an analyte into a sample inlet of a sample analysis device, the sample analysis device comprising a plurality of channels constructed and arranged to receive the sample suspected of containing an analyte from the sample inlet, each channel comprising a different chemical reagent, each chemical reagent capable of generating a luminescent or colorimetric signal; treating the sample with a plurality of different solvents provided from a corresponding plurality of solvent chambers disposed within the sample analysis device, wherein the plurality of solvent chambers are in fluid communication with the sample inlet; and simultaneously introducing the treated sample into the plurality of channels such that the analyte, if present, interacts with one or more of the chemical reagents to produce a plurality of luminescent or colorimetric signals, thereby determining the analyte, wherein the plurality of channels are in fluid communication with the sample inlet, and wherein the presence and/or identity of the analyte is determined without need for a non-luminescent or non-colorimetric analysis of the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method for determining an analyte, comprising:
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contacting a surface of a rolling substrate of a sample collection device with an article suspected of containing an analyte via a rolling or tapping motion such that a sample suspected of containing the analyte is collected on the surface of the rolling substrate; contacting the surface of the rolling substrate with a fluid carrier to transfer at least a portion of the sample to the fluid carrier to produce a fluid sample suspected of containing the analyte; introducing the fluid sample suspected of containing the analyte into a sample inlet of a sample analysis device comprising a plurality of channels constructed and arranged to receive the fluid sample; treating the fluid sample with a plurality of solvents provided from a corresponding plurality of solvent chambers disposed within the sample analysis device, wherein the plurality of solvent chambers are in fluid communication with the sample inlet; simultaneously introducing portions of the fluid sample into each of a set of the plurality of channels, each of the set of channels comprising a different chemical reagent capable of generating a luminescent and/or colorimetric signal upon interaction with a specific analyte, wherein the analyte, if present in the fluid sample, interacts with one or more of the chemical reagents to produce a plurality of luminescent and/or colorimetric signals in at least some of the set of channels, wherein the plurality of channels are in fluid communication with the sample inlet; and determining the presence and/or identity of the analyte based on the plurality of luminescent and/or colorimetric signals.
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24. A kit for determining an analyte, comprising:
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a sample collection device, comprising; a first region comprising a fluid container containing a fluid carrier; a second region comprising a surface constructed and arranged to contact an article suspected of containing the analyte and to receive fluid from the fluid container; a sample outlet in fluid communication with the first and second regions; and a housing containing the first region, the second region, and the sample outlet; and a sample analysis device, comprising; at least one sample inlet constructed and arranged to receive a sample suspected of containing an analyte from the sample outlet of the sample collection device; a plurality of solvent chambers disposed within the sample analysis device and providing a plurality of different solvents for dissolving the sample, wherein the plurality of solvent chambers are in fluid communication with the at least one sample inlet; and a channel in fluid communication with the at least one sample inlet and configured to receive the dissolved sample, the channel comprising a chemical reagent capable of generating a luminescent or colorimetric signal.
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Specification