Lateral flow assays using two dimensional features
First Claim
1. A test device for detecting an analyte in a liquid sample, which device comprises a plurality of reagent dots on a matrix, wherein said plurality of reagent dots do not overlap and are sufficiently spaced apart from each other so that when said liquid sample flows laterally along said matrix, flow of said liquid sample to, through and/or around one of said plurality of reagent dots does not substantially affect flow of said liquid sample to, through and/or around said other reagent dots, said plurality of reagent dots do not form a reagent line across the entire width of said matrix in a direction perpendicular to the direction of said liquid sample flow or a complete circle of a reagent line, and after a liquid sample flows laterally along said test device and passes said plurality of reagent dots, said plurality of reagent dots form a predetermined pattern to indicate presence, absence and/or amount of said analyte in said liquid sample,wherein said plurality of reagent dots comprise different binding reagents that are capable of binding to different analytes or other binding reagents that are capable of binding to the analytes, said plurality of reagent dots form a line that is substantially parallel to the liquid sample flow direction, as the liquid sample flows laterally along the test device, the analytes, if present in the liquid sample, become bound to the binding reagents at each of said plurality of reagent dots, the binding of the analytes to said reagent dots generates detectable signals at said reagent dots, and the presence and/or intensity of the detectable signals at said reagent dots indicates the presence and/or amount of the analytes in the liquid sample, andwherein at least a quarter, a third, half or all of said reagent dots do not overlap and are sufficiently spaced apart from each other so that when the liquid sample flows laterally along the matrix, flow of the liquid sample to, through and/or around one of the reagent dots does not substantially affect flow of the liquid sample to, through and/or around the other reagent dots.
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Accused Products
Abstract
The present invention relates to novel lateral flow devices using two dimensional features, preferably, uniform two dimensional test and control features, and the methods for detecting an analyte using the lateral flow devices, and processes for making the lateral flow devices.
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Citations
22 Claims
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1. A test device for detecting an analyte in a liquid sample, which device comprises a plurality of reagent dots on a matrix, wherein said plurality of reagent dots do not overlap and are sufficiently spaced apart from each other so that when said liquid sample flows laterally along said matrix, flow of said liquid sample to, through and/or around one of said plurality of reagent dots does not substantially affect flow of said liquid sample to, through and/or around said other reagent dots, said plurality of reagent dots do not form a reagent line across the entire width of said matrix in a direction perpendicular to the direction of said liquid sample flow or a complete circle of a reagent line, and after a liquid sample flows laterally along said test device and passes said plurality of reagent dots, said plurality of reagent dots form a predetermined pattern to indicate presence, absence and/or amount of said analyte in said liquid sample,
wherein said plurality of reagent dots comprise different binding reagents that are capable of binding to different analytes or other binding reagents that are capable of binding to the analytes, said plurality of reagent dots form a line that is substantially parallel to the liquid sample flow direction, as the liquid sample flows laterally along the test device, the analytes, if present in the liquid sample, become bound to the binding reagents at each of said plurality of reagent dots, the binding of the analytes to said reagent dots generates detectable signals at said reagent dots, and the presence and/or intensity of the detectable signals at said reagent dots indicates the presence and/or amount of the analytes in the liquid sample, and wherein at least a quarter, a third, half or all of said reagent dots do not overlap and are sufficiently spaced apart from each other so that when the liquid sample flows laterally along the matrix, flow of the liquid sample to, through and/or around one of the reagent dots does not substantially affect flow of the liquid sample to, through and/or around the other reagent dots.
Specification