Cell washing device using standing acoustic waves and a phantom material

• US 9,855,382 B2
• Filed: 03/31/2015
• Issued: 01/02/2018
• Est. Priority Date: 03/31/2015
• Status: Active Grant

• Current Assignee: Biomet Manufacturing, LLC (Zimmer Biomet Holdings, Inc.)
• Original Assignee: Biomet Biologics LLC (Zimmer Biomet Holdings, Inc.)
• Inventors: Leach, Michael D., Hamman, Ned M., Abeskaron, David, Cunningham, Grant D., Dorian, Randel, Storrs, Richard W., King, Scott R.
• Primary Examiner(s): MARCETICH, ADAM M
• Application Number:
  US14/674,089
• Publication Number:
  US 20160287778A1
• Time in Patent Office: 1,008 Days
• CPC Class Codes: A61K 35/18 Erythrocytes haemoglobin A61K38/42
• CPC Class Codes: A61M 1/0281 Apparatus for treatment of blood or blood constituents prior to transfusion, e.g. washing, filtering or thawing
• CPC Class Codes: A61M 1/3678 Separation of cells using wave pressure; Manipulation of individual corpuscles
• CPC Class Codes: A61M 1/3692 Washing or rinsing blood or blood constituents
• CPC Class Codes: A61M 2202/0429 Red blood cells; Erythrocytes
• CPC Class Codes: A61M 2205/3375 Acoustical, e.g. ultrasonic, measuring means
• CPC Class Codes: B01D 21/283 Settling tanks provided with vibrators
• CPC Class Codes: B01D 43/00 Separating particles from liquids, or liquids from solids, otherwise than by sedimentation or filtration flotation processes B03D1/00; drying solid materials or objects F26B
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0436 acoustic forces, e.g. surface acoustic waves [SAW]
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 5/0641 Erythrocytes

Abstract

Devices for washing a composition including cells are provided. The devices include a separation channel, a first wave component and a second wave component. The separation channel is positioned between the first and second wave components. Collectively, the wave components generate a standing bulk acoustic wave that isolates cells from the composition.

Claims

1. A device for washing a composition comprising red blood cells, the device comprising:

a body having a first surface, a second opposing surface, a first side, a second opposing side, a first end, and a second end, the body defining a channel extending along a longitudinal axis from the first end to the second end, wherein the channel comprises a separation region and a mixing region, the body further including a first planar shelf proximate the first end and arranged to isolate a first inflow stream from a second inflow stream prior to the first inflow stream and the second inflow stream entering the mixing region;

a first sidewall located along the first side within the channel and extending along the longitudinal axis;

a first protrusion extending from the first surface and adjacent to the first sidewall, the first protrusion extending a length of the first sidewall;

a second sidewall located along the second side within the channel and extending along the longitudinal axis, the first sidewall and the second sidewall being a phantom material;

a second protrusion extending from the first surface and adjacent to the second sidewall, the second protrusion extending a length of the second sidewall;

a first wave component coupled to the first surface; and

a second wave component coupled to the second surface,

wherein the separation region of the channel is positioned between the first wave component and the second wave component, and wherein the first wave component and second wave component are configured to generate a first standing acoustic wave.

2. The device according to claim 1, wherein the first wave component is a first wave generator and the second wave component is either a second wave generator, a reflective material, a reflective surface, or a reflective layer.

3. The device according to claim 2, wherein one of the first or second wave components is a reflective surface formed as the first or second surface.

4. The device according to claim 1, wherein a portion of the first surface and the second surface is composed of the phantom material.

5. The device according to claim 4, wherein the phantom material mimics acoustical properties of water.

6. The device according to claim 1, comprising a first inlet for delivering the first inflow stream and a second inlet for delivering the second inflow stream positioned on the second surface near the first end, and a first outlet and a second outlet positioned on the second surface near the second end, wherein the inlets and outlets are in fluid communication with the channel.

7. The device according to claim 6, wherein the device is configured to have the composition introduced into the device through the first inlet and a wash material introduced into the device through the second inlet.

8. The device according to claim 7, wherein the wash material is selected from the group consisting of water, saline, dextrose, saline with 5% dextrose, phosphate buffered saline.

9. The device according to claim 8, wherein the device is configured such that the composition and the wash material are mixed together after being introduced to the device and passing the first planar shelf, and then separated from each other in the separation region of the channel by a pressure node associated with the first standing acoustic wave.

10. A device for washing a multicomponent mixture, the device comprising:

a body having a first surface, and a second opposing surface;

a first wave generator coupled to the first surface;

a wave component selected from the group consisting of a second wave generator, a first reflective material, a first reflective surface, and a first reflective layer, wherein the wave component is coupled to or forms the second surface;

wherein the body defines a channel having a channel floor, channel walls, and a channel ceiling, the channel extending along the longitudinal axis from a first end region of the device to a second end region of the device, the channel comprising a receiving region near the first end region, a collection region near the second end region, and a separation region between the receiving region and collection region, the separation region being positioned between the first wave generator and the wave component, the receiving region divided into a first inflow region and a second inflow region by a first shelf, the collection region divided into a first outflow region and a second outflow region by a second shelf,

wherein a first protrusion extends along and is adjacent to a first one of the channel walls within the channel and a second protrusion extends along and is adjacent to a second one of the channel walls within the channel, the first protrusion and the second protrusion extending from the channel floor to the channel ceiling, and

wherein the channel floor, the first protrusion, the second protrusion, and the channel walls comprise a phantom material.

11. The device according to claim 10, wherein the first wave generator and the wave component generate a standing bulk acoustic wave with a pressure node located in the separation region of the channel.

12. The device according to claim 10, further comprising:

a first inlet in fluid communication with the first inflow region and the mixing region;

a second inlet in fluid communication with the second inflow region and the mixing region;

a first outlet in fluid communication with the first outflow region and the collection region; and

a second outlet in fluid communication with the second outflow region and the collection region.

13. The device according to claim 12, wherein the device is configured to receive the multicomponent mixture comprising red blood cells through the first inlet and a wash material through the second inlet, wherein the multi component mixture and the wash material are mixed at the mixing region of the channel.

14. The device according to claim 13, wherein the first wave generator and the second wave generator or first reflective material generate a standing bulk acoustic wave with a pressure node positioned in the separation region of the channel, and the pressure node separates the red blood cells from the wash solution.

15. The device according to claim 14, wherein the red blood cells exit the device through the first outlet and the wash material exits the device through the second outlet.

16. The device according to claim 10, wherein the phantom material mimics the acoustic properties of water.

17. The device according to claim 10, wherein the separation channel has a length of from about 20 mm to about 100 mm, a width from about 5 mm to about 50 mm, and a height of from about 0.5 mm to about 3 mm.

18. A device for washing a composition comprising red blood cells, the device comprising:

a plurality of layers defining a channel having a mixing region, a separation region, and a collection region, a first layer of the plurality of layers extending into the mixing region and isolating a first inlet from a second inlet, the first inlet and the second inlet open to the mixing region, the mixing region open to the separation region, the separation region in fluid communication with the collection region;

a first sidewall located within the channel along a first side of the channel and extending along a longitudinal axis of the channel;

a first protrusion extending from a bottom layer of the plurality of layers to a top layer of the plurality of layers and along and adjacent to the first sidewall;

a second sidewall located within the channel along a second side of the channel and extending along the longitudinal axis, the first sidewall and the second sidewall being a phantom material;

a second protrusion extending from the bottom layer of the plurality of layers to the top layer of the plurality of layers and along and adjacent to the second sidewall;

a first wave component coupled to a first surface of the channel; and

a second wave component coupled to a second surface of the channel, the second surface parallel to the first surface,

wherein the separation region of the channel is positioned between the first wave component and the second wave component, and wherein the first wave component and second wave component are configured to generate a first standing acoustic wave.

19. The device according to claim 18, wherein the first wave component is a first wave generator and the second wave component is either a second wave generator, a reflective material, a reflective surface, or a reflective layer.

20. The device according to claim 18, wherein one of the first or second wave components is a reflective surface formed as the first or second surface.

21. The device according to claim 18, wherein a portion of at least one of the first surface or the second surface is composed of the phantom material.

22. The device according to claim 21, wherein the phantom material mimics acoustical properties of water.