Please download the dossier by clicking on the dossier button x
×

Chimeric proteins and methods of regulating gene expression

  • US 9,856,497 B2
  • Filed: 01/10/2017
  • Issued: 01/02/2018
  • Est. Priority Date: 01/11/2016
  • Status: Active Grant
First Claim
Patent Images

1. A system for regulating expression of a target polynucleotide in a cell, the system comprising:

  • (a) a chimeric receptor polypeptide comprising a transmembrane receptor or a fragment thereof, that undergoes a receptor modification including a conformational change or chemical modification upon binding to its ligand, wherein the chimeric receptor polypeptide comprises a cleavage moiety that cleaves a cleavage recognition site; and

    (b) a chimeric adaptor polypeptide that binds the chimeric receptor polypeptide in response to the receptor modification, wherein the chimeric adaptor polypeptide comprises a gene modulating polypeptide (GMP) comprising an actuator moiety linked to the cleavage recognition site;

    wherein the cleavage moiety cleaves the cleavage recognition site when in proximity to the cleavage recognition site and in response to receptor binding to the ligand, and wherein upon cleaving the cleavage recognition site, the actuator moiety regulates expression of the target polynucleotide.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×