Transparent and flexible neural electrode arrays

• US 9,861,288 B2
• Filed: 07/11/2014
• Issued: 01/09/2018
• Est. Priority Date: 07/11/2014
• Status: Active Grant

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Ma, Zhenqiang, Williams, Justin Cole, Park, Dong-Wook, Schendel, Amelia Ann, Mikael, Solomon Tadesse
• Primary Examiner(s): Cohen, Lee S
• Application Number:
  US14/329,067
• Publication Number:
  US 20160007874A1
• Time in Patent Office: 1,278 Days
• Field of Search: 600377, 600378
• CPC Class Codes: A61B 2503/40 Animals
• CPC Class Codes: A61B 2503/42 for laboratory research
• CPC Class Codes: A61B 2562/046 in a matrix array
• CPC Class Codes: A61B 2562/125 characterised by the manufacture of electrodes
• CPC Class Codes: A61B 5/24 Detecting, measuring or recording bioelectric or biomagnetic signals of the body or parts thereof
• CPC Class Codes: A61B 5/291 for electroencephalography [EEG]
• CPC Class Codes: A61B 5/6868 Brain

Abstract

Devices for detecting electrical activity in electrically active biological tissues and methods for using the devices are provided. The devices include an electrode array that is configured for implantation on electrically active biological tissue. The electrode array comprises a plurality of electrode sites comprising one or more layers of transparent, electrically conductive graphene disposed on a transparent substrate.

Claims

1. A device for detecting electrical activity in electrically active biological tissue, the device comprising:

a micro-electrode array configured for implantation on the electrically active biological tissue and comprising;

a biocompatible, dielectric substrate;

a plurality of electrode sites on the biocompatible, dielectric substrate, the electrode sites comprising one or more sheets of electrically conductive graphene, wherein the one or more sheets of electrically conductive graphene are non-planar sheets, and further wherein the non-planar sheets of graphene comprise wrinkled graphene or crumpled graphene;

a plurality of electrically conductive contacts on the biocompatible, dielectric substrate; and

electrically conductive interconnects connecting the electrode sites to the contacts;

wherein the electrode sites and the portion of the substrate on which the electrode sites are disposed are transparent in at least a portion of the wavelength range from about 300 to about 2000 nm.

2. The device of claim 1, wherein the substrate is a mechanically flexible polymeric substrate.

3. The device of claim 2, wherein the substrate comprises a shape memory polymer.

4. The device of claim 1, wherein the number of electrically conductive sheets of graphene in the electrode sites is in the range from 1 to 10.

5. The device of claim 1, wherein the electrically active biological tissue is neural tissue and the device further comprises an electronic device electrically connected to the contacts that is configured to receive neural signals recorded at the electrode sites and to amplify, display, store or analyze the neural signals.

6. The device of claim 1, wherein the graphene around the perimeters of the electrode sites comprises wrinkled graphene or crumpled graphene and the graphene in the center regions of the electrode sites comprises planar graphene.

7. A method of detecting electrical signals in electrically active biological tissue using the device of claim 1, the method comprising:

implanting the micro-electrode array on the electrically active biological tissue; and

recording an electrical signal generated in the electrically active biological tissue at the electrode sites.

8. The method of claim 7, wherein the tissue is muscle tissue.

9. The method of claim 7, wherein the tissue is cardiac tissue.

10. The method of claim 7, wherein the tissue is neural tissue.

11. The method of claim 10, wherein the electrical signal is a neural response generated by neural cells in the neural tissue, the method further comprising exposing the neural tissue to a sensory stimulus to evoke the neural response.

12. The method of claim 11, wherein the neural cells incorporate light-sensitive proteins and the sensory stimulus comprises light directed onto the neural tissue through the electrode sites, the light comprising wavelengths that photostimulate the light-sensitive proteins.

13. The method of claim 11, wherein the neural tissue is fluorescently labeled, the method further comprising directing ultraviolet light onto the neural tissue through the electrode sites to induce the emission of fluorescence from the fluorescently labeled tissue and recording the emitted fluorescence.

14. The method of claim 11, wherein the neural tissue is the surface of a cerebral cortex.

15. The method of claim 14, further comprising exposing the cerebral cortex to a sensory stimulus to evoke the generation of action potentials, wherein the action potentials are the neural response.

16. The method of claim 15, wherein the neural cells incorporate light-sensitive proteins and the sensory stimulus comprises light directed onto the surface of the cerebral cortex through the electrode sites, the light comprising wavelengths that photostimulate the light-sensitive proteins.

17. The method of claim 14, wherein the cerebral cortex comprises fluorescently labeled tissue, the method further comprising directing ultraviolet light onto the cerebral cortex through the electrode sites to induce the emission of fluorescence from the fluorescently labeled tissue and recording the emitted fluorescence.

18. The method of claim 14, further comprising directing infrared light onto the cerebral cortex through the electrode sites and recording backscattered light from the cerebral cortex.

19. A method of detecting backscattered light from neural tissue using the device of claim 1,

the method comprising:

implanting the micro-electrode array on the neural tissue; and

directing infrared light onto the neural tissue through the electrode sites and recording backscattered light from the neural tissue.

20. The method of claim 19, wherein the infrared light directed onto the neural tissue through the electrode sites comprises wavelengths in the range from 1300 nm to 2000 nm.