Method and apparatus for measuring concentration of test substance in organism

• US 9,861,303 B2
• Filed: 02/25/2015
• Issued: 01/09/2018
• Est. Priority Date: 01/25/2013
• Status: Active Grant

• Current Assignee: Panasonic Intellectual Property Management Co., Ltd. (Panasonic Holdings Corporation)
• Original Assignee: Panasonic Intellectual Property Management Co., Ltd. (Panasonic Holdings Corporation)
• Inventors: Kawamura, Tatsurou, Okumura, Yasuaki, Minamiguchi, Masaru, Shioi, Masahiko
• Primary Examiner(s): Winakur, Eric
• Assistant Examiner(s): Liu, Chu Chuan (JJ)
• Application Number:
  US14/631,095
• Publication Number:
  US 20150164393A1
• Time in Patent Office: 1,049 Days
• CPC Class Codes: A61B 5/1451 for interstitial fluid
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons

Abstract

An exemplary apparatus for measuring the concentration of a test substance in an organism disclosed herein includes: a measurement system which measures first and second concentrations that are concentrations of the test substance at positions A and B, respectively, where the positions A and B are located inside of the organism but outside of the blood vessel of the organism, and the position B is located more distant from the blood vessel than the position A is; and a decision circuit which determines, based on the first and second concentrations, whether or not an equilibrium has been established yet between a concentration of the test substance inside of the blood vessel and a concentration of the test substance measured at a position inside of the organism but outside of the blood vessel of the organism.

Claims

1. An apparatus for measuring the concentration of a test substance in an organism, the apparatus comprising:

a first sensor which is configured to be arranged at a position A, the position A is located inside of the organism but outside of a blood vessel of the organism;

a second sensor which is configured to be arranged at a position B, the position B is located inside of the organism but outside of the blood vessel of the organism, and is located more distant from the blood vessel than the position A;

an irradiation unit which irradiates the first and second sensors with irradiating light;

a detector which detects a first returning light beam that has returned from around the first sensor and a second returning light beam that has returned from around the second sensor;

a calculation circuit which calculates first and second concentrations based on the first and second returning light beams, respectively, and the first and second concentrations are concentrations of the test substance at the positions A and B, respectively; and

a decision circuit which determines, based on the first and second concentrations, whether or not an equilibrium has been established yet between a concentration of the test substance inside of the blood vessel and a concentration of the test substance measured at a position inside of the organism but outside of the blood vessel of the organism,

wherein when the difference between the first and second concentrations is less than a predetermined value, the decision circuit decides that the equilibrium has already been established between the concentration of the test substance inside of the blood vessel and the concentration of the test substance measured at the position inside of the organism but outside of the blood vessel of the organism.

2. The apparatus of claim 1, wherein when the decision circuit decides that the equilibrium has already been established, a measured value of the first concentration is output as the concentration of the test substance in the organism.

3. The apparatus of claim 1, wherein when the difference between the first and second concentrations is equal to or greater than a predetermined value, the decision circuit decides that the equilibrium has not been established yet between the concentration of the test substance inside of the blood vessel and the concentration of the test substance measured at the position inside of the organism but outside of the blood vessel of the organism.

4. The apparatus of claim 3, wherein when the decision circuit decides that the equilibrium has not been established yet, notification is made that the concentration of the test substance in the blood vessel is now changing steeply.

5. The apparatus of claim 3, wherein when the decision circuit decides that the equilibrium has not been established yet, a measured value of the first concentration is output provisionally as a concentration of the test substance in the organism, and

notification is made that the measured value of the first concentration output is a provisional value.

6. The apparatus of claim 3, further comprising a heater,

wherein when the decision circuit decides that the equilibrium has not been established yet, the heater heats a region surrounding the positions A and B.

7. The apparatus of claim 1, wherein in each of the first and second sensors, a metal pattern has been formed on a side of the first and second sensors to be irradiated with the irradiating light,

the irradiating light is light which induces localized surface plasmon resonance in the metal pattern,

the first returning light beam is a first surface enhanced Raman scattered light beam which has been produced in the vicinity of the metal pattern of the first sensor,

the first concentration is calculated based on the radiant intensity of the first surface enhanced Raman scattered light beam,

the second returning light beam is a second surface enhanced Raman scattered light beam which has been produced in the vicinity of the metal pattern of the second sensor, and

the second concentration is calculated based on the radiant intensity of the second surface enhanced Raman scattered light beam.

8. The apparatus of claim 7, wherein the first and second surface enhanced Raman scattered light beams are surface enhanced Raman scattered light beams which have been produced by the test substance that is present in the vicinity of the metal pattern.

9. The apparatus of claim 7, wherein a trapping substance to trap the test substance is immobilized on the respective metal patterns of the first and second sensors.

10. The apparatus of claim 9, wherein the first and second surface enhanced Raman scattered light beams are surface enhanced Raman scattered light beams which have been produced by the trapping substance that is present in the vicinity of the metal pattern.

11. The apparatus of claim 1, wherein the first sensor is a first fluorescent fine particle,

the second sensor is a second fluorescent fine particle,

the first and second fluorescent fine particles produce fluorescent light, of which the radiant intensity changes in reaction to the test substance in the organism, when irradiated with the irradiating light,

the irradiating light is light, of which the wavelength is tuned to the absorption wavelength of the first and second fluorescent fine particles,

the first returning light beam is a first fluorescent light beam which has been produced in the vicinity of the first fluorescent fine particle,

the first concentration is calculated based on the radiant intensity of the first fluorescent light beam,

the second returning light beam is a second fluorescent light beam which has been produced in the vicinity of the second fluorescent fine particle, and

the second concentration is calculated based on the radiant intensity of the second fluorescent light beam.

12. The apparatus of claim 1, wherein the first and second sensors are configured to be embedded in the dermis of the organism.

13. The apparatus of claim 1, wherein the test substance is glucose.

14. The apparatus of claim 1, wherein the position B is configured to be located shallower in a depth direction of the organism than the position A.

15. A method for measuring the concentration of a test substance in an organism, the method comprising the steps of:

(a) measuring first and second concentrations using first and second sensors, respectively, that are concentrations of the test substance at positions A and B, respectively, wherein the first sensor is configured to be arranged at the position A, the position A is located inside of the organism but outside of a blood vessel of the organism, and the second sensor is configured to be arranged at the position B, the position B is located inside of the organism but outside of the blood vessel of the organism, and is located more distant from the blood vessel than the position A;

(b) irradiating, via an irradiation unit, the first and second sensors with irradiating light;

(c) detecting, via a detector, a first returning light beam that has returned from around the first sensor and a second returning light beam that has returned from around the second sensor;

(d) calculating, via a calculation circuit, the first and second concentrations based on the first and second returning light beams, respectively, and the first and second concentrations are concentrations of the test substance at the positions A and B, respectively; and

(e) determining, via a decision circuit, based on the first and second concentrations, whether or not an equilibrium has been established yet between a concentration of the test substance inside of the blood vessel and a concentration of the test substance measured at a position inside of the organism but outside of the blood vessel of the organism,

wherein when the difference between the first and second concentrations is less than a predetermined value, the decision circuit decides that the equilibrium has already been established between the concentration of the test substance inside of the blood vessel and the concentration of the test substance measured at the position inside of the organism but outside of the blood vessel of the organism.

16. The method of claim 15, wherein the position B is configured to be located shallower in a depth direction of the organism than the position A.