Specificity, flexibility and valence of DNA bonds for guided emulsion architecture

• US 9,861,569 B2
• Filed: 04/30/2014
• Issued: 01/09/2018
• Est. Priority Date: 05/01/2013
• Status: Active Grant

• Current Assignee: New York University
• Original Assignee: New York University
• Inventors: Brujic, Jasna, Feng, Lang, Pontani, Lea-Laetitia, Chaikin, Paul
• Primary Examiner(s): EPPS -SMITH, JANET L
• Application Number:
  US14/266,026
• Publication Number:
  US 20140330002A1
• Time in Patent Office: 1,350 Days
• Field of Search: 536 231, 536 253, 424450, 424490
• CPC Class Codes: A23L 29/10 containing emulsifiers
• CPC Class Codes: A23L 35/10 Emulsified foodstuffs
• CPC Class Codes: A61K 2800/413 Nanosized, i.e. having sizes below 100 nm
• CPC Class Codes: A61K 2800/57 Compounds covalently linked to a(n inert) carrier molecule, e.g. conjugates, pro-fragrances
• CPC Class Codes: A61K 8/0241 Containing particulates characterized by their shape and/or structure see also A61K8/04, A61K8/11, and A61K8/14, further aspects are classified in A61K2800/40 and subcodes
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin

Abstract

A method of forming an end product by self-assembly of a first component having a patch of a linker component, such as DNA strands, cadherins, adhesive proteins and nanoparticle linkers. Such emulsions can be used to process personal care products, skin cremes, foods and animal feedstocks.

Claims

1. A method of self-assembly to form end product, comprising the steps of:

providing a first emulsion droplet for assembly;

providing a second emulsion droplet for assembly;

providing a first linker comprising a colloidal nanoparticle which can be coupled to the first emulsion droplet;

forming a first patch, comprising the first linker, between the first emulsion droplet and the second emulsion droplet.

2. The method as defined in claim 1 wherein pseudo-bonding valency is established for the linker components.

3. The method as defined in claim 2 wherein a valency of 2 enables formation of flexible polymers of emulsion droplets.

4. The method as defined in claim 2 wherein a pseudo-valency of 4 enables formation of rigid polymer networks.

5. The method as defined in claim 1 wherein the first linker further comprises a plurality of single stranded DNA, attached to the colloidal nanoparticle, having a first sequence and further wherein the first emulsion droplet and the second emulsion droplet each have attached thereto a plurality of single stranded DNA having a second sequence, the second sequence and the first sequence being complementary.

6. The method as defined in claim 1 further including the step of mixing the end production with a personal care product matrix.

7. The method as defined in claim 1 further including the step of mixing the end product with a food product matrix.

8. The method as defined in claim 1 wherein the end production consists of an amorphous material having selectable rheological properties, thereby enabling processing of the end product to operate consumer products having desired properties.

9. The method as defined in claim 1 further including the step of cycling temperature of the end product.

10. The method as defined in claim 1 wherein the linker component comprises DNA strands further including the step of increased coverage of the DNA strands on the first component, thereby enabling tuning of reversibility of self-assembly.

11. A method of self-assembly to form end product, comprising the steps of:

providing an emulsion having a plurality of emulsion droplets and a plurality of linkers, wherein each linker comprises a nanoparticle having attached thereto a first DNA strand and a second DNA strand and the concentration of the plurality of linkers is selected to provide a desired valency;

providing a first linker and a second linker of the plurality of linkers, each of the first linker and the second linker engagable with the plurality of emulsion droplets and engageable with each other;

engaging the first DNA strand with a first emulsion droplet of the plurality of droplets;

engaging the second DNA strand with a second emulsion droplet of the plurality of droplets;

forming a patch between the first emulsion droplet and the second emulsion droplet comprising the first DNA strand, the nanoparticle, and the second DNA strand;

engaging the first emulsion droplet with additional emulsion droplets to achieve the desired valency and

creaming the emulsion forming a floppy network of the plurality of emulsion droplets interconnected by a plurality of patches.

12. The method of claim 11, wherein the emulsion has a ratio of 5 to 1 of the plurality of linkers to the plurality of droplets, whereby a linear assembly is formed.

13. The method of claim 11, wherein the emulsion has a ratio of 100 to 1 of the plurality of linkers to the plurality of emulsion droplets, whereby a folded assembly is formed.

14. The method of claim 11, further comprising selecting a valency for the floppy network.