Device and method for particle complex handling

• US 9,863,857 B2
• Filed: 07/31/2014
• Issued: 01/09/2018
• Est. Priority Date: 12/29/2006
• Status: Active Grant

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Su, Xing, Liu, David J., Wu, Kai, Swartz, Kenneth B., Yamakawa, Mineo
• Primary Examiner(s): Reifsnyder, David A
• Application Number:
  US14/448,141
• Publication Number:
  US 20140342470A1
• Time in Patent Office: 1,258 Days
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0424 Dielectrophoretic forces
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: G01N 1/4044 by chemical techniques; Digestion; Chemical decomposition
• CPC Class Codes: G01N 2015/0038 Investigating nanoparticles
• CPC Class Codes: G01N 2015/0092 Monitoring flocculation or agglomeration
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: H01F 2007/068 using printed circuit coils
• CPC Class Codes: H01F 7/20 without armatures cores H01F3/00; coils H01F5/00 ; shaping metal by applying magnetic forces B21D26/14; electromagnets specially adapted for NMR applications G01R33/381
• CPC Class Codes: H01F 7/206 Electromagnets for lifting, handling or transporting of magnetic pieces or material electromagnets for guidance of vehicles, workpieces B65G21/2009; for magnetic suspension or levitation H02N15/00
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10T 29/49117 Conductor or circuit manufacturing
• CPC Class Codes: Y10T 29/4913 : Assembling to base an electrical component, e.g., capacitor, etc.
• CPC Class Codes: Y10T 29/49155 : Manufacturing circuit on or in base
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

Claims

1. A method comprising providing a device comprising an integrated circuitry component and a fluidic network comprising a magnetic particle in a fluid, generating a magnetic field that interacts with the fluidic network, and transporting the magnetic particle by the magnetic field that interacts with the fluidic network without fluidic movement of the fluid in the fluidic network, wherein generating the magnetic field that interacts with the fluidic network is by programmably activating magnetic microcoils in a vicinity of the fluidic network.

2. The method of claim 1, wherein the magnetic particle comprises a magnetic affinity complex bound to an analyte.

3. The method of claim 1, wherein the magnetic particle comprises a magnetic affinity complex and a signal affinity complex, both bound to different bind sites of an analyte.

4. The method of claim 3, wherein the analyte is a protein, an antibody, or a nucleic acid.

5. The method of claim 1, wherein the magnetic particle comprises a signal affinity complex, bound to an analyte.

6. The method of claim 5, further comprising detecting the analyte bound to the magnetic particle.

7. The method of claim 1, wherein the magnetic particle comprises a coded affinity complex.

8. The method of claim 7, wherein the coded affinity complex is not magnetic.

9. The method of claim 1, further comprising vibrating the fluid.

10. The method of claim 1, wherein transporting the magnetic particle comprises transporting the magnetic particle through a diffusion barrier that is part of the fluidic network.