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Methods for repairing degraded DNA

  • US 9,873,906 B2
  • Filed: 10/07/2010
  • Issued: 01/23/2018
  • Est. Priority Date: 07/14/2004
  • Status: Expired due to Fees
First Claim
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1. A kit comprising a purified endonuclease, a purified DNA N-glycosylase, a purified AP lyase, a purified DNA polymerase, a purified 3′

  • -diesterase, a purified polynucleotide kinase, a purified large Klenow fragment, a purified DNA ligase, a mixture of random primers comprising an equal part mix of hexamers, heptamers, and octamers with thiophosphate linkages for the last two 3′

    nucleotides and a mixture of deoxynucleotide triphosphates, wherein the kit optionally further includes a highly processive strand displacing polymerase.

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