Methods for repairing degraded DNA
First Claim
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1. A kit comprising a purified endonuclease, a purified DNA N-glycosylase, a purified AP lyase, a purified DNA polymerase, a purified 3′
- -diesterase, a purified polynucleotide kinase, a purified large Klenow fragment, a purified DNA ligase, a mixture of random primers comprising an equal part mix of hexamers, heptamers, and octamers with thiophosphate linkages for the last two 3′
nucleotides and a mixture of deoxynucleotide triphosphates, wherein the kit optionally further includes a highly processive strand displacing polymerase.
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Abstract
The present invention provides methods and kits for repair of degraded DNA which may then be used as a template for efficient amplification by a number of different amplification reactions. The method relies upon a series of enzymatic activities provided by DNA repair enzymes.
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Citations
7 Claims
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1. A kit comprising a purified endonuclease, a purified DNA N-glycosylase, a purified AP lyase, a purified DNA polymerase, a purified 3′
- -diesterase, a purified polynucleotide kinase, a purified large Klenow fragment, a purified DNA ligase, a mixture of random primers comprising an equal part mix of hexamers, heptamers, and octamers with thiophosphate linkages for the last two 3′
nucleotides and a mixture of deoxynucleotide triphosphates, wherein the kit optionally further includes a highly processive strand displacing polymerase. - View Dependent Claims (2, 3, 4, 5, 6, 7)
- -diesterase, a purified polynucleotide kinase, a purified large Klenow fragment, a purified DNA ligase, a mixture of random primers comprising an equal part mix of hexamers, heptamers, and octamers with thiophosphate linkages for the last two 3′
Specification
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Current AssigneeIbis Biosciences Incorporated (Abbott Laboratories)
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Original AssigneeIbis Biosciences Incorporated (Abbott Laboratories)
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InventorsEshoo, Mark W.
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Primary Examiner(s)Bertagna, Angela M
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Assistant Examiner(s)OYEYEMI, OLAYINKA A
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Application NumberUS12/900,104Publication NumberTime in Patent Office2,665 DaysField of Search435 612, 435810US Class CurrentCPC Class CodesC12Q 1/6806 Preparing nucleic acids for...C12Q 2521/301 EndonucleaseC12Q 2521/501 LigaseC12Q 2521/514 Mismatch repair proteinC12Q 2521/531 Glycosylase
