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Signal detection techniques for the detection of analytes

  • US 9,874,542 B2
  • Filed: 10/14/2014
  • Issued: 01/23/2018
  • Est. Priority Date: 09/17/1998
  • Status: Expired due to Fees
First Claim
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1. A method of detecting a target nucleic acid in a sample on a biochip, said method comprising:

  • a) providing a biochip comprising detection electrodes;

    b) contacting the detection electrodes of said biochip with said sample in the presence of a label probe specifically hybridizable to said target nucleic acid under conditions permitting hybridization of said label probe to said target nucleic acid, said label probe comprising a covalently attached electrochemical detection label comprising a ferrocene derivative;

    c) performing a wash step to remove non-specific binding of said label probe to said biochip;

    d) applying an input electric signal to said biochip;

    e) detecting an output electric signal from said biochip, compared with an output electric signal obtained from said method which is performed when the target is not present, the presence of higher amplitudes of harmonic peaks from said output signal at different higher harmonic frequencies indicates the presence of said target nucleic acid in the sample.

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