Aptamer-based in vivo diagnostic system

• US 9,874,554 B1
• Filed: 07/16/2014
• Issued: 01/23/2018
• Est. Priority Date: 07/16/2014
• Status: Active Grant

• Current Assignee: Verily Life Sciences LLC (Alphabet Inc.)
• Original Assignee: Verily Life Sciences LLC (Alphabet Inc.)
• Inventors: Schwartz, Jerrod Joseph, Bajaj, Vikram Singh, Thompson, Jason Donald
• Primary Examiner(s): Negin, Russell S
• Application Number:
  US14/333,161
• Time in Patent Office: 1,287 Days
• Field of Search: None
• CPC Class Codes: A61B 5/0515 Magnetic particle imaging
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/681 Wristwatch-type devices
• CPC Class Codes: G01N 33/54333 Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction

Abstract

A system for modulating a response signal includes aptamer conjugates, e.g., aptamer-particle conjugates, configured to bind with target analytes, a detector configured to detect an analyte response signal transmitted from the body, a modulation source configured to modulate the analyte response signal, and a processor configured to non-invasively detect the one or more target analytes by differentiating the analyte response signal from a background signal, at least in part, based on the modulation. The analyte response signal is related to the binding interaction of the target analytes with the aptamer-particle conjugates. In some examples, the system may also include magnetic particles and a magnetic field source sufficient to distribute the magnetic particles into a spatial arrangement in the body. The analyte response signal may be differentiated from the background signal, at least in part, based on modulation of the signals due, at least in part, to the spatial arrangement of the magnetic particles.

Claims

1. A system comprising:

aptamer-particle conjugates, wherein the aptamer-particle conjugates can bind with one or more target analytes present in an environment;

a detector;

magnetic particles;

a magnetic field source;

a processor; and

a computer readable medium storing instructions executable by the processor to perform operations comprising;

controlling the detector to detect a response signal transmitted from the environment, wherein the response signal includes a background signal and an analyte response signal related to binding of the one or more target analytes with the aptamer-particle conjugates;

controlling the magnetic field source to distribute the magnetic particles into a spatial arrangement in the environment, wherein the spatial arrangement of the magnetic particles can modulate the analyte response signal, such that the analyte response signal is affected differently than the background signal; and

non-invasively detecting the one or more target analytes by differentiating the analyte response signal from the background signal based, at least in part, on modulation of the analyte response signal, wherein the analyte response signal is modulated differently than the background signal or modulated differently than an unbound particle signal.

2. The system of claim 1, wherein the unbound particle signal is related to aptamer-particle conjugates that are not bound with the one or more target analytes.

3. The system of claim 2, wherein the processor can further non-invasively detect the one or more target analytes by differentiating the analyte response signal from the unbound particle signal, at least in part, based on modulation of the signals due, at least in part, to the spatial arrangement of the magnetic particles.

4. The system of claim 3, wherein the analyte response signal is modulated differently than the unbound particle signal.

5. The system of claim 2, further comprising an interrogating signal source, the response signal being transmitted in response to the interrogating signal.

6. The system of claim 2, wherein the environment comprises a fluid conduit, a fluid reservoir or a living body.

7. The system of claim 1, wherein the environment comprises a living body and further comprising a wearable device having a mount to mount the wearable device to an external surface of the living body, wherein the detector is mounted on the wearable device.